en
jalali
1396
11
1
gregorian
2018
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1
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online
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fulltext
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Association between Long Noncoding RNA ANRIL Expression Variants and Susceptibility to Coronary Artery Disease
Animal cells possess thousands of long non-coding (lnc) RNAs, such as antisense noncoding RNA in the INK4 locus (ANRIL) , which have regulatory roles in the cells’ molecular mechanisms, including X-chromosome inactivation, and developmental processes. These lnc RNAs are known to influence the extensive spectrum of age-related disorders. Accordingly, there is evidence for the role of these lnc RNAs in cardiovascular diseases, particularly coronary artery diseases (CAD). The aim of this study was to assess whether the expression of the lnc RNA ANRIL was associated with a susceptibility to CAD by evaluating the expression level of the two transcripts of ANRIL. Peripheral blood was taken from fifty patients affected by CAD and relative expression of ANRIL was determined by Real-Time PCR assay. The obtained data indicated that the EU741058 transcript expression level was significantly decreased in CAD patients in comparison with the healthy individuals (P= 0.001). Furthermore, there was no significant association between the NR_003529 transcript expression, and CAD risk in Iranian patients (P= 0.751). Our results suggest that the expression level of the EU741058 transcript of ANRIL may be implicated in CAD development, creating a predictive biomarker for CAD patients in future.
Coronary artery disease, atherosclerosis, long noncoding RNA, ANRIL, chromosome 9p21
1
7
http://ijmcmed.org/browse.php?a_code=A-10-1291-1&slc_lang=en&sid=1
2017/09/15
1396/6/24
2018/01/6
1396/10/16
Mohsen
Yari
Department of Medical Genetics, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
mohsen.yari.m@gmail.com
00319475328460011368
00319475328460011368
No
Sara
Bitarafan
Department of Medical Genetics, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
00319475328460011369
00319475328460011369
No
Mohammad Ali
Broumand
Department of Molecular Pathology, Tehran Heart Center, Tehran University of Medical Sciences, Tehran, Iran.
00319475328460011370
00319475328460011370
No
Zahra
Fazeli
Department of Medical Genetics, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
00319475328460011371
00319475328460011371
No
Mahnoosh
Rahimi
Department of Medical Genetics, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
00319475328460011372
00319475328460011372
No
Sayyed Mohammad Hossein
Ghaderian
Department of Medical Genetics, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
00319475328460011373
00319475328460011373
No
Reza
Mirfakhraie
Department of Medical Genetics, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
00319475328460011374
00319475328460011374
No
Mir Davood
Omrani
Department of Medical Genetics, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
davood_omrani@sbmu.ac.ir
00319475328460011375
00319475328460011375
Yes
en
Downregulation of Matrix Metalloproteinases 2 and 9 is Involved in the Protective Effect of Trehalose on Spinal Cord Injury
Upregulation of matrix metalloproteinases (MMPs), in particular MMP-2 and MMP-9 contributes to secondary pathogenesis of spinal cord injury (SCI) via promoting inflammation. Recently, we reported that trehalose suppresses inflammatory responses following SCI. Therefore, we investigated the effect of trehalose on MMP-2 and MMP-9 expression in SCI. A weight-drop contusion SCI was induced in male rats. Then, animals received trehalose at three doses of 10 (T10), 100 (T100) and 1000 (T1000) mM intrathecally. MMP-2 and MMP-9 transcripts were then measured in damaged spinal cord at 1, 3 and 7 days after trauma, and compared with vehicle and sham groups. Additionally, behavioral analysis was conducted for 1 week using Basso-Beattie-Bresnahan (BBB) locomotor rating scale. Our data showed an early upregulation of MMP-9 at 1 day post-SCI. However, MMP-2 expression was increased at 3 days after trauma. Treatment with 10 mM trehalose significantly reduced MMP-2 expression at 3 and 7 days (P< 0.01) and MMP-9 expression at 1, 3, and 7 days (P< 0.05) post-damage compared with vehicle. However, downregulation of both MMPs was not observed in T100 and T1000 groups. In addition, T10 group showed more rapid recovery of hind limb strength compared with T100 and T1000 groups. We propose that neuroprotective effect of low dose trehalose is mediated by attenuation of MMP-2 and MMP-9 expression.
Spinal cord injury, trehalose, matrix metalloproteinases
8
16
http://ijmcmed.org/browse.php?a_code=A-10-1327-1&slc_lang=en&sid=1
2017/09/152017/12/6
1396/9/15
2018/01/62018/03/17
1396/12/26
Masoumeh
Mirzaie
Department of Clinical Biochemistry, Afzalipour School of Medicine, Kerman University of Medical Sciences, Kerman, Iran.
masoumeh.0511@gmail.com
00319475328460011376
00319475328460011376
No
Mehrnaz
Karimi
Department of Clinical Biochemistry, Afzalipour School of Medicine, Kerman University of Medical Sciences, Kerman, Iran.
mehrnaz.karimi6878@gmail.com
00319475328460011377
00319475328460011377
No
Hossein
Fallah
Department of Clinical Biochemistry, Afzalipour School of Medicine, Kerman University of Medical Sciences, Kerman, Iran.
hofallah2014@gmail.com
00319475328460011378
00319475328460011378
No
Mohammad
Khaksari
Endocrinology and Metabolism Research Center, Institute of Basic and Clinical Physiology Sciences, Kerman University of Medical Sciences, Kerman, Iran.
khaksar38@yahoo.co.uk
00319475328460011379
00319475328460011379
No
Mahdieh
Nazari-Robati
Neuroscience Research Center, Institute of Neuropharmacology and Department of Clinical Biochemistry, Afzalipour School of Medicine, Kerman University of Medical Sciences, Kerman, Iran.
mnazari@kmu.ac.ir
00319475328460011380
00319475328460011380
Yes
en
A Comprehensive Genetic and Clinical Evaluation of Waardenburg Syndrome Type II in a Set of Iranian Patients
Waardenburg syndrome (WS) is a neurocristopathy with an autosomal dominant mode of inheritance, and considerable clinical and genetic heterogeneity. WS type II is the most common type of WS in many populations presenting with sensorineural hearing impairment, heterochromia iridis, hypoplastic blue eye, and pigmentary abnormalities of the hair and skin. To date, mutations of MITF, SOX10, and SNAI2 have been implicated in the pathogenesis of WS2. Although different pathogenic mutations have been reported in many ethnic groups, the data on Iranian WS2 patients is insufficient. 31 WS2 patients, including 22 men and 9 women from 14 families were included. Waardenburg consortium guidelines were employed for WS2 diagnosis. WS2 patients underwent screening for MITF, SOX10, and SNAI2 mutations using direct sequencing and MLPA analysis. Clinical evaluation revealed prominent phenotypic variability in Iranian WS2 patients. Sensorineural hearing impairment and heterochromia iridis were the most common features (67% and 45%, respectively), whereas anosmia was the least frequent phenotype. Molecular analysis revealed a de novo heterozygous c.640C>T (p.R214X) in MITF and a de novo heterozygous SOX10 gross deletion in the study population. Our data help illuminate the phenotypic and genotypic spectrum of WS2 in an Iranian series of patients, and could have implications for the genetic counseling of WS in Iran.
Waardenburg syndrome type 2, Iran, MLPA, gene deletion, mutation
17
23
http://ijmcmed.org/browse.php?a_code=A-10-1356-1&slc_lang=en&sid=1
2017/09/152017/12/62018/01/6
1396/10/16
2018/01/62018/03/172018/03/23
1397/1/3
Nazanin
Jalilian
Department of Clinical biochemistry, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran.
n.jalilian@kums.ac.ir
00319475328460011381
00319475328460011381
No
Mohammad Amin
Tabatabaiefar
Department of Genetics and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
mamintab@yahoo.co.uk
00319475328460011382
00319475328460011382
No
Mahboubeh
Yazdanpanah
Department of Medical Genetics, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
yazdanpanah_iryas@yahoo.com
00319475328460011383
00319475328460011383
No
Elham
Darabi
Department of Medical Genetics, School of Medicine, International Campus, Tehran University of Medical Sciences, Tehran, Iran.
eli.darly@gmail.com
00319475328460011384
00319475328460011384
No
Tayyeb
Bahrami
Department of Medical Genetics, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
tayyeb_bahrami@yahoo.com
00319475328460011385
00319475328460011385
No
Ali
Zekri
Department of Medical Genetics and Molecular Biology, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran.
azekri87@gmail.com
00319475328460011386
00319475328460011386
No
Mohammad Reza
Noori-Daloii
Department of Medical Genetics, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
nooridaloii@tums.ac.ir
00319475328460011387
00319475328460011387
Yes
en
Overexpression of MiR-138 Inhibits Cell Growth and Induces Caspase-mediated Apoptosis in Acute Promyelocytic Leukemia Cell Line
Dysregulated expression of miRNAs can play a vital role in pathogenesis of leukemia. The shortened telomere length, and elevated telomerase activity in acute promyelocytic leukemia cells are mainly indicative of extensive proliferative activity. This study aimed to investigate the effect of overexpression of miR-138 on telomerase activity, and cell proliferation of acute promyelocytic leukemia NB4 cells. MiR-138 was overexpressed in NB4 cells using GFP hsa-miR-138-expressing lentiviruses. hTERT mRNA and protein expression levels were assessed by qRT-PCR and western blot analysis. For evaluation of apoptosis, annexin-V staining and activation of caspases were assessed using flow cytometry and western blot analysis, respectively. Our data demonstrate that overexpression of miR-138 attenuated the hTERT mRNA and protein expression levels. In addition, cell growth was inhibited, and malignant cells underwent caspase mediated-apoptosis in response to miR-138 overexpression. These findings suggest that loss of miR-138 expression may be associated with increased telomerase activity in NB4 cells. Therefore, strategies for up-regulation of miR-138 may result in inhibition of malignant cell growth, and provide a promising therapeutic approach for acute promyelocytic leukemia.
Apoptosis, caspase, hTERT, miR-138, poly ADP ribose polymerase (PARP)
24
31
http://ijmcmed.org/browse.php?a_code=A-10-987-2&slc_lang=en&sid=1
2017/09/152017/12/62018/01/62018/01/27
1396/11/7
2018/01/62018/03/172018/03/232018/03/26
1397/1/6
Rima
Manafi shabestari
Department of Hematology and Blood Banking, Faculty of Allied Medicine, Iran University of Medical Sciences, Tehran, Iran.
manafirima@gmail.com
00319475328460011388
00319475328460011388
No
Fatemeh
Alikarami
Department of Hematology and Blood Banking, Faculty of Allied Medicine, Iran University of Medical Sciences, Tehran, Iran.
fatemehalikarami43@gmail.com
00319475328460011389
00319475328460011389
No
Davood
Bashash
Department of Hematology and Blood Banking, School of Allied Medical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
d.bashash@sbmu.ac.ir
00319475328460011390
00319475328460011390
No
Mostafa
Paridar
Ministry of Health and Medical Education, Deputy of Management and Resources Development, Tehran, Iran.
mstparidar@gmail.com
00319475328460011391
00319475328460011391
No
Majid
Safa
Department of Hematology and Blood Banking, Faculty of Allied Medicine, Iran University of Medical Sciences, Tehran, Iran.
safa.m@iums.ac.ir
00319475328460011392
00319475328460011392
Yes
en
Epidemiology of Infantile Visceral Leishmaniasis in Western Algerian And The Convenience of Serum For The Disease Diagnosis by PCR and Immunochromatography
Epidemiological situation of infantile visceral leishmaniasis (IVL), which is a public health problem in Algeria, is almost unknown in the cities of Western part of the country. The aim of this study was to analyze the epidemiological, clinical, biological, therapeutic, and evolutionary aspects of IVL in Western Algeria, to evaluate the performance of the immunochromatography as a rapid diagnostic test of the disease, and to propose a diagnosis approach by real-time polymerase chain reaction (RT-PCR) assay from the serum. This prospective study was performed on 63 suspicious cases of visceral leishmaniasis collected from the infectious diseases department at the Pediatric Hospital of Oran from January 2012 to July 2017. For each patient, the epidemiological parameters, and the clinical and biological data were collected. Bone marrow and blood samples were drawn from all cases. Bone marrow was performed to research amastigote forms of Leishmania and to identify the species by PCR-sequencing. Blood samples were used to detect anti-Leishmania antibodies as well as parasite DNA. Patients from the Western regions were mostly from rural areas. Sensitivity of RT-PCR from the bone marrow and from serum was 95.45% and 94.44%, respectively. The immunochromatography allowed the disease’s diagnosis for 11 cases whose myelogram did not confirm the presence of the amastigote forms of Leishmania. Immunochromatography was revealed to be a good technique for disease diagnosis regarding the strongly evocative clinical signs. The results also suggest the interest of the RT-PCR assay from patient serum as a non-invasive sample, in the detection of parasite DNA.
Imunochromatography, RT-PCR, bone marrow, serum, sequencing, Leishmania infantum
32
43
http://ijmcmed.org/browse.php?a_code=A-10-1311-1&slc_lang=en&sid=1
2017/09/152017/12/62018/01/62018/01/272017/12/2
1396/9/11
2018/01/62018/03/172018/03/232018/03/262018/02/14
1396/11/25
Touria
Hadj-Slimane
Natural and Life Sciences Faculty, Department of Biology, University of Oran 1 Ahmed Ben Bella, Oran, Algeria.
hadjslimanetouria@gmail.com
00319475328460011393
00319475328460011393
Yes
kheira
Senouci
Natural and Life Sciences Faculty, Department of Biology, University of Oran 1 Ahmed Ben Bella, Oran, Algeria.
kheirasenouci@yahoo.fr
00319475328460011394
00319475328460011394
No
Nori
Midoun
Department of Epidemiology and Preventive Medicine, University Hospital of Oran (EHU), Oran, Algeria.
semepehuo@gmail.com
00319475328460011395
00319475328460011395
No
Assia
Bouchetara
Infectious Diseases Department, Pediatric Hospital of Oran (EHS), Oran, Algeria.
bouchetaraa@gmail.com
00319475328460011396
00319475328460011396
No
Amel
Laradj
Infectious Diseases Department, Pediatric Hospital of Oran (EHS), Oran, Algeria.
ameldj81@yahoo.fr
00319475328460011397
00319475328460011397
No
Fadi
Bittar
Aix-Marseille University- Faculty of Pharmacy, IHU-Méditerranée Infection, Marseille, France.
fadi.BITTAR@univ-amu.fr
00319475328460011398
00319475328460011398
No
en
Marine Actinomycetes with Probiotic Potential and Bioactivity Against Multidrug-resistant Bacteria
Considering antimicrobial resistance problem, marine microorganisms with the bioactivity against multi-drug resistant (MDR) pathogens have attracted many scientific interests. To address this issue, a total of 21 marine actinomycetes isolated from the Caspian Sea have been screened out. Primary screening via cross-streak method revealed that 3 strains: MN2, MN39, and MN40 produce antimicrobial agents with wide spectrum activity. In the second step, the potent strains were characterized morphologically, and then identified genetically using 16S rRNA analysis. After that, the bioactivity of the ethyl acetate extracts of liquid culture against some MDR bacteria has been studied using disc diffusion method. Finally, the exoenzymatic activity of the strains, and the anti-vibrio activity of the extracts have been evaluated. The nucleotide sequence of the 16S rRNA gene (1.5 kb) showed that the potent strains belong to the genus Streptomyces. The results of disk diffusion method indicated that among the 3 potent isolates, MN39 and MN2 produce biomolecules with antibacterial activity against MDR bacteria specially methicillin-resistant Staphylococcus aureus (MRSA) and vancomycin-resistant Enterococcus (VRE). In addition, potent strains showed remarkable anti-vibrio activity as well as extracellular enzyme production including amylase and protease. The results of this study revealed that the marine actinomycetes isolated from the sediments of Caspian Sea produce biomolecules effective against MDR bacteria, and suggested that these strains deserve to be studied as potential probiotics due to their anti-vibrio activity besides exoenzyme production.
Marine actinomycetes, antimicrobial activity, Caspian Sea, multi-drug resistant bacteria, antivibrio
44
52
http://ijmcmed.org/browse.php?a_code=A-10-1352-1&slc_lang=en&sid=1
2017/09/152017/12/62018/01/62018/01/272017/12/22017/12/31
1396/10/10
2018/01/62018/03/172018/03/232018/03/262018/02/142018/03/29
1397/1/9
Hamed
Norouzi
Department of Biology, University of Isfahan, Isfahan, Iran.
hamed.nt68@yahoo.com
00319475328460011399
00319475328460011399
No
Abolghasem
Danesh
Biotechnology Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran.
danesha@mums.ac.ir
00319475328460011400
00319475328460011400
No
Mojtaba
Mohseni
Department of Microbiology, University of Mazandaran, Babolsar, Iran.
m.mohseni@umz.ac.ir
00319475328460011401
00319475328460011401
No
Mohammad
Rabbani khorasgani
Department of Biology, University of Isfahan, Isfahan, Iran.
m.rabbani@biol.ui.ac.ir
00319475328460011402
00319475328460011402
Yes
en
In silico Homology Modeling and Epitope Prediction of NadA as a Potential Vaccine Candidate in Neisseria meningitidis
Neisseria meningitidis is a facultative pathogen bacterium which is well founded with a number of adhesion molecules to facilitate its colonization in human nasopharynx track. Neisseria meningitidis is a major cause of mortality from sever meningococcal disease and septicemia. The Neisseria meningitidis adhesion, NadA, is a trimeric autotransporter adhesion molecule which is involved in cell adhesion, invasion, and antibody induction. It is identified in approximately 50% of N. meningitidis isolates, and is established as a vaccine candidate due to its antigenic effects. In the present study we exploited bioinformatics tools to better understand and determine the 3D structure of NadA and its functional residues to select B cell epitopes, and provide information for elucidating the biological function and vaccine efficacy of NadA. Therefore, this study provided essential data to close gaps existing in biological areas. The most appropriate model of NadA was designed by SWISS MODEL software and important residues were determined using the subsequent epitope mapping procedures. Locations of important linear and conformational epitopes were determined and conserved residues were identified to broaden our knowledge of efficient vaccine designing to reduce meningococcal infectious in population. These data now provide a theme to design more broadly cross-protective antigens.
Neisseria meningitidis, NadA, epitope prediction, 3D structure
53
68
http://ijmcmed.org/browse.php?a_code=A-10-1207-1&slc_lang=en&sid=1
2017/09/152017/12/62018/01/62018/01/272017/12/22017/12/312017/07/5
1396/4/14
2018/01/62018/03/172018/03/232018/03/262018/02/142018/03/292018/01/24
1396/11/4
Narjes
Shahsavani
Shahid Sadoughi University of Medical Sciences and Health Services, Yazd, Iran.
narjesshahsavani@gmail.com
00319475328460011403
00319475328460011403
No
Mohammad Hasan
Sheikhha
Shahid Sadoughi University of Medical Sciences and Health Services, Yazd, Iran.
sheikhha@ssu.ac.ir
00319475328460011404
00319475328460011404
No
Hasan
Yousefi
Department of Medical Genetics, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
Hassanmbi@yahoo.com
00319475328460011405
00319475328460011405
No
Fatemeh
Sefid
Department of Biology, Science and Art University, Yazd, Iran.
sefid@shahed.ac.ir
00319475328460011406
00319475328460011406
Yes