@article{ author = {Caruso, Maddalena and Evangelista, Marco and Parolini, Ornell}, title = {Human term placental cells: phenotype, properties and new avenues in regenerative medicine}, abstract ={The human placenta has long been the subject of scientific interest due to the important roles which it performs during pregnancy in sustaining the fetus and maintaining fetomaternal tolerance. More recently, however, researchers have begun to investigate the possibility that the placenta’s utility may extend beyond fetal development to act as a source of cells with clinically relevant properties. Indeed, several groups have reported the isolation of cells from different placental regions which display both multilineage differentiation potential and immunomodulatory properties in vitro. Furthermore, these cells have also been shown to secrete soluble factors involved in pathophysiological processes that may aid tissue repair. Cells with such features will clearly find application in the field of regenerative medicine for the repair/regeneration of damaged or diseased tissues or organs. In line with these promising findings, several preclinical and clinical studies conducted to date argue in strong favor of the therapeutic utility of placenta-derived cells for the treatment of several diseases. Although much work remains to be conducted in order to fully understand the properties of placental cells and the mechanisms which underlie their beneficial effects in vivo, data reported to date nonetheless provide compelling evidence in support of the placenta as a cell source for use in regenerative medicine.}, Keywords = {Amnion, chorion, placenta, regenerative medicine, stem cells}, volume = {1}, Number = {2}, pages = {64-74}, publisher = {Babol University of Medical Sciences}, url = {http://ijmcmed.org/article-1-39-en.html}, eprint = {http://ijmcmed.org/article-1-39-en.pdf}, journal = {International Journal of Molecular and Cellular Medicine (IJMCM)}, issn = {2251-9637}, eissn = {2251-9645}, year = {2012} } @article{ author = {Mulherkar, Rita and Husain, Tabish}, title = {Lymphoblastoid cell lines: a continuous in vitro source of cells to study carcinogen sensitivity and DNA repair}, abstract ={Obtaining a continuous source of normal cells or DNA from a single individual has always been a rate limiting step in biomedical research. Availability of Lymphoblastoid cell lines (LCLs) as a surrogate for isolated or cryopreserved peripheral blood lymphocytes has substantially accelerated the process of biological investigations. LCLs can be established by in vitro infection of resting B cells from peripheral blood with Epstein Barr Virus (EBV) resulting in a continuous source, bearing negligible genetic and phenotypic alterations. Being a spontaneous replicating source, LCLs fulfil the requirement of constant supply of starting material for variety of assays, sparing the need of re-sampling. There is a reason to believe that LCLs are in close resemblance with the parent lymphocytes based on the ample supporting observations from a variety of studies showing significant level of correlation at molecular and functional level. LCLs, which carry the complete set of germ line genetic material, have been instrumental in general as a source of biomolecules and a system to carry out various immunological and epidemiological studies. Furthermore, in recent times their utility for analysing the whole human genome has extensively been documented. This proves the usefulness of LCLs in various genetic and functional studies. There are a few contradictory reports that have questioned the employment of LCLs as parent surrogate. Regardless of some inherent limitations LCLs are increasingly being considered as an important resource for genetic and functional research.}, Keywords = {Lymphoblastoid cell lines, Epstein Barr virus, cell immortalization, carcinogen sensitivity, DNA damage/repair }, volume = {1}, Number = {2}, pages = {75-87}, publisher = {Babol University of Medical Sciences}, url = {http://ijmcmed.org/article-1-28-en.html}, eprint = {http://ijmcmed.org/article-1-28-en.pdf}, journal = {International Journal of Molecular and Cellular Medicine (IJMCM)}, issn = {2251-9637}, eissn = {2251-9645}, year = {2012} } @article{ author = {Nazarpour, Robab and Zabihi, Ebrahim and Alijanpour, Ebrahim and Abedian, Zeinab and Mehdizadeh, Hamid and Rahimi, Fatemeh}, title = {Optimization of human peripheral blood mononuclear cells (PBMCs) cryopreservation}, abstract ={Cryopreservation is the method of choice for long term storage of human PBMCs. This study was designed to compare the different combinations of variables affecting the cryopreservation of PBMCs samples. The viability of PBMCs separated from 2×5 ml peripheral blood samples obtained from 16 healthy adult volunteers, were measured using trypan blue dye exclusion method just before freezing with different concentrations of DMSO (10, 15, and 20%) and FBS (40 or 70%) at two different temperatures (either 4oC or 25oC). Then after 2 weeks the cells were thawed and the viability was measured again. Also the PBMCs response to PHA was measured after 48 h using MTT assay. The effects of the different variables were calculated and compared among the groups. A total of 192 PBMCs cryotubes made from blood samples of 16 volunteers were tested. The viability of the cells obtained by the two centrifugation procedure was the same (both more than 99%). The concentration of the FBS (40 vs 70%) did not show to have significant effects on either cells viability or response to PHA. On the other hand 20% DMSO concentration and freezing temperature at 25oC decreased both cells. Based on the obtained results, it is recommended to centrifuge the PBMCs under higher revolt speed at shorter time (700 g for 20 minutes) and decrease the FBS concentration to 40%. The DMSO concentration should be kept at 10-15% and the freezing medium be cooled down to 4oC.}, Keywords = {PBMCs, ficol, freezing, DMSO, FBS}, volume = {1}, Number = {2}, pages = {88-93}, publisher = {Babol University of Medical Sciences}, url = {http://ijmcmed.org/article-1-48-en.html}, eprint = {http://ijmcmed.org/article-1-48-en.pdf}, journal = {International Journal of Molecular and Cellular Medicine (IJMCM)}, issn = {2251-9637}, eissn = {2251-9645}, year = {2012} } @article{ author = {Shokravi, Marziyeh and Tabarsi, Bahareh and Moghaddamnia, Aliakbar and Sohanfaraji, Alieh and Pourghasem, Mohse}, title = {Comparison of skin reaction between MTA (produced in Iran) and CEM in Rabbit}, abstract ={Pathological changes in pulp and periapical tissues are addressed by endodontic treatment. The material used in this treatment must be biocompatible. The aim of this study is to compare the skin reaction of Calcium Enriched Mixture (CEM) and Mineral Trioxide Aggregate (MTA) produced in Iran on rabbit. Sixteen male newzeland albino rabbits weighting 2 kg were used. The animals back hair was shaved, 24 hours before application of each material. The material was applied on two sites (2 × 2 cm) while the third site was used as control. All sites were covered by gauze and bandaged for 4 hours. Then the material's remnants were washed off the sites of application. Observations were performed in 1, 24, 48 and 72 hours after removing the materials. Erythematous surface areas were measured by the morphometric method. After sacrificing animals the skins were dissected and the specimens were prepared for histological evaluation. There were significant differences between CEM and MTA in erythematous surface areas at 1, 24 and 48 hours after removing the materials (p0.05). The average erythematous surface areas were wider in MTA sites than CEM sites. As a conclusion it seems that biocompatibility of CEM could be more than MTA.}, Keywords = {Skin reaction , MTA made in Iran, CEM}, volume = {1}, Number = {2}, pages = {94-98}, publisher = {Babol University of Medical Sciences}, url = {http://ijmcmed.org/article-1-44-en.html}, eprint = {http://ijmcmed.org/article-1-44-en.pdf}, journal = {International Journal of Molecular and Cellular Medicine (IJMCM)}, issn = {2251-9637}, eissn = {2251-9645}, year = {2012} } @article{ author = {Hassanzadeh-Nazarabadi, Mohammad and Shekouhi, Sahar and Seif, Najmeh}, title = {The incidence of spontaneous abortion in mothers with blood group O compared with other blood types}, abstract ={Although ABO incompatibility between mother and fetus has long been suspected as cause of spontaneous abortion in man, its precise contribution has not been completely resolved. In spite of reports in which the incompatible mating was recognized to be a cause of habitual abortion, and which eventually results in infertility or a reduction in the number of living children compared with the number in compatible matings, such effects were not observed in other studies. The aim of this review article was to show some evidence of relationship between ABO incompatibility and spontaneous abortion.}, Keywords = {spontaneous abortion, ABO blood group, incompatibility }, volume = {1}, Number = {2}, pages = {99-104}, publisher = {Babol University of Medical Sciences}, url = {http://ijmcmed.org/article-1-40-en.html}, eprint = {http://ijmcmed.org/article-1-40-en.pdf}, journal = {International Journal of Molecular and Cellular Medicine (IJMCM)}, issn = {2251-9637}, eissn = {2251-9645}, year = {2012} } @article{ author = {Dasar, Negin and Ghaderian, Sayyed Mohammad Hossein and Azargashb, Eznollah}, title = {Human Evaluation of the Glu298Asp polymorphism in NOS3 gene and its relationship with onset age of ESRD in Iranian patients suffering from ADPKD}, abstract ={One of the most striking features in autosomal dominant polycystic kidney disease (ADPKD) is the difference at onset age of end-stage renal disease (ESRD). Modifier genes may play a role in this phenotypic variability. The mutated nitric oxide synthase 3 gene (NOS3), have a modifier effect on the severity of ADPKD by impairment of NOS3 activity and decreasing of renal vascular nitric oxide production and, subsequently, reduced kidney function. In order to test this hypothesis, we investigated the relationship between Glu298Asp polymorphism in exon 7 of this gene and ESRD in ADPKD patients refered from Shahid Labbafi Nedjad Hospital in Tehran. The polymorphism was examined by PCR, followed by RFLP (with MboI) in three groups of ADPKD with ESRD ADPKD without ESRD patients and normal individual as the cases, case-controls and controls, respectively. The frequencies of GG, GT, and TT genotypes in cases were 66.7%, 33.3% and 0%, in case-controls were 78.6%, 19%, 2.4%, and in controls were 64.3%, 35.7% and 0%, respectively. Our findings revealed that there was no significant difference in the genotype frequency of NOS3 gene in ADPKD patients (p=0.311).The age of onset of ESRD in ADPKD patients, harbouring the T allele of this polymorphism, was two years lower than G/G patients, but this difference was not significant (p =0.641). In conclusion, our results suggest that there is no evidence of relationship between Glu298Asp polymorphism and onset age of ESRD in Iranian ADPKD patients.}, Keywords = {ADPKD, ESRD, NOS3, Glu298Asp polymorphism}, volume = {1}, Number = {2}, pages = {105-112}, publisher = {Babol University of Medical Sciences}, url = {http://ijmcmed.org/article-1-49-en.html}, eprint = {http://ijmcmed.org/article-1-49-en.pdf}, journal = {International Journal of Molecular and Cellular Medicine (IJMCM)}, issn = {2251-9637}, eissn = {2251-9645}, year = {2012} } @article{ author = {KholghiOskooei, Vahid and EsmaeiliDooki, Mohammad Reza and Akhavan-Niaki, Haleh}, title = {Analysis of c.3369+213TA[7-56] and D7S523 microsatellites linked to Cystic Fibrosis Transmembrane Regulator.}, abstract ={  Cystic fibrosis (CF) is a life-limiting autosomal recessive disorder affecting principally respiratory and digestive system . It is caused by cystic fibrosis transmembrane conductance regulator (CFTR) gene mutation. The aim of this study was to determine the extent of repeat numbers and the degree of heterozygosity for c.3499+200TA(7_56) and D7S523 located in intron 17b and 1 cM proximal to the CFTR gene respectively. Both microsatellites were analyzed by direct electrophoresis of PCR product on 20% polyacrylamide gel in 40 Normal subjects and 40 CF patients originating from North Iran. 9 different alleles were found for D7S523 ranging from 16 to 24 repeats alleles. (CA)20 was the most prevalent allele both in normal individuals and CF patients with 21.3% and 20% frequencies respectively. Heterozygosity frequency of D7S523 in normal individuals and CF patients was 97.5% and 90% respectively. Eighteen different alleles were found for c.3499+200TA(7_56) ranging from 8 to 38 repeats alleles. (TA)9 was the most prevalent allele both in normal individuals and CF patients with 30% and 23.5% frequencies respectively. All normal subjects and 97.5% of CF patients showed heterozyous genotype. The high heterozygosity of the two studied microsatellites witnesses the dynamism of such markers. High degree of heterozygosity of c.3499+200TA(7_56) and D7S523 make these markers, a very useful tool for prenatal diagnosis especially in Iranian population.}, Keywords = {Cystic Fibrosis, c.3369+213TA[7-56], D7S523, Iran}, volume = {1}, Number = {2}, pages = {113-118}, publisher = {Babol University of Medical Sciences}, url = {http://ijmcmed.org/article-1-51-en.html}, eprint = {http://ijmcmed.org/article-1-51-en.pdf}, journal = {International Journal of Molecular and Cellular Medicine (IJMCM)}, issn = {2251-9637}, eissn = {2251-9645}, year = {2012} }