eng
Babol University of Medical Sciences
International Journal of Molecular and Cellular Medicine (IJMCM)
2251-9637
2251-9645
2018-02
7
1
1
7
article
Association between Long Noncoding RNA ANRIL Expression Variants and Susceptibility to Coronary Artery Disease
Mohsen Yari
mohsen.yari.m@gmail.com
1
Sara Bitarafan
2
Mohammad Ali Broumand
3
Zahra Fazeli
4
Mahnoosh Rahimi
5
Sayyed Mohammad Hossein Ghaderian
6
Reza Mirfakhraie
7
Mir Davood Omrani
davood_omrani@sbmu.ac.ir
8
Department of Medical Genetics, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Department of Medical Genetics, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Department of Molecular Pathology, Tehran Heart Center, Tehran University of Medical Sciences, Tehran, Iran.
Department of Medical Genetics, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Department of Medical Genetics, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Department of Medical Genetics, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Department of Medical Genetics, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Department of Medical Genetics, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Animal cells possess thousands of long non-coding (lnc) RNAs, such as antisense noncoding RNA in the INK4 locus (ANRIL) , which have regulatory roles in the cells’ molecular mechanisms, including X-chromosome inactivation, and developmental processes. These lnc RNAs are known to influence the extensive spectrum of age-related disorders. Accordingly, there is evidence for the role of these lnc RNAs in cardiovascular diseases, particularly coronary artery diseases (CAD). The aim of this study was to assess whether the expression of the lnc RNA ANRIL was associated with a susceptibility to CAD by evaluating the expression level of the two transcripts of ANRIL. Peripheral blood was taken from fifty patients affected by CAD and relative expression of ANRIL was determined by Real-Time PCR assay. The obtained data indicated that the EU741058 transcript expression level was significantly decreased in CAD patients in comparison with the healthy individuals (P= 0.001). Furthermore, there was no significant association between the NR_003529 transcript expression, and CAD risk in Iranian patients (P= 0.751). Our results suggest that the expression level of the EU741058 transcript of ANRIL may be implicated in CAD development, creating a predictive biomarker for CAD patients in future.
http://ijmcmed.org/article-1-739-en.pdf
Coronary artery disease
atherosclerosis
long noncoding RNA
ANRIL
chromosome 9p21
eng
Babol University of Medical Sciences
International Journal of Molecular and Cellular Medicine (IJMCM)
2251-9637
2251-9645
2018-02
7
1
8
16
article
Downregulation of Matrix Metalloproteinases 2 and 9 is Involved in the Protective Effect of Trehalose on Spinal Cord Injury
Masoumeh Mirzaie
masoumeh.0511@gmail.com
1
Mehrnaz Karimi
mehrnaz.karimi6878@gmail.com
2
Hossein Fallah
hofallah2014@gmail.com
3
Mohammad Khaksari
khaksar38@yahoo.co.uk
4
Mahdieh Nazari-Robati
mnazari@kmu.ac.ir
5
Department of Clinical Biochemistry, Afzalipour School of Medicine, Kerman University of Medical Sciences, Kerman, Iran.
Department of Clinical Biochemistry, Afzalipour School of Medicine, Kerman University of Medical Sciences, Kerman, Iran.
Department of Clinical Biochemistry, Afzalipour School of Medicine, Kerman University of Medical Sciences, Kerman, Iran.
Endocrinology and Metabolism Research Center, Institute of Basic and Clinical Physiology Sciences, Kerman University of Medical Sciences, Kerman, Iran.
Neuroscience Research Center, Institute of Neuropharmacology and Department of Clinical Biochemistry, Afzalipour School of Medicine, Kerman University of Medical Sciences, Kerman, Iran.
Upregulation of matrix metalloproteinases (MMPs), in particular MMP-2 and MMP-9 contributes to secondary pathogenesis of spinal cord injury (SCI) via promoting inflammation. Recently, we reported that trehalose suppresses inflammatory responses following SCI. Therefore, we investigated the effect of trehalose on MMP-2 and MMP-9 expression in SCI. A weight-drop contusion SCI was induced in male rats. Then, animals received trehalose at three doses of 10 (T10), 100 (T100) and 1000 (T1000) mM intrathecally. MMP-2 and MMP-9 transcripts were then measured in damaged spinal cord at 1, 3 and 7 days after trauma, and compared with vehicle and sham groups. Additionally, behavioral analysis was conducted for 1 week using Basso-Beattie-Bresnahan (BBB) locomotor rating scale. Our data showed an early upregulation of MMP-9 at 1 day post-SCI. However, MMP-2 expression was increased at 3 days after trauma. Treatment with 10 mM trehalose significantly reduced MMP-2 expression at 3 and 7 days (P< 0.01) and MMP-9 expression at 1, 3, and 7 days (P< 0.05) post-damage compared with vehicle. However, downregulation of both MMPs was not observed in T100 and T1000 groups. In addition, T10 group showed more rapid recovery of hind limb strength compared with T100 and T1000 groups. We propose that neuroprotective effect of low dose trehalose is mediated by attenuation of MMP-2 and MMP-9 expression.
http://ijmcmed.org/article-1-780-en.pdf
Spinal cord injury
trehalose
matrix metalloproteinases
eng
Babol University of Medical Sciences
International Journal of Molecular and Cellular Medicine (IJMCM)
2251-9637
2251-9645
2018-02
7
1
17
23
article
A Comprehensive Genetic and Clinical Evaluation of Waardenburg Syndrome Type II in a Set of Iranian Patients
Nazanin Jalilian
n.jalilian@kums.ac.ir
1
Mohammad Amin Tabatabaiefar
mamintab@yahoo.co.uk
2
Mahboubeh Yazdanpanah
yazdanpanah_iryas@yahoo.com
3
Elham Darabi
eli.darly@gmail.com
4
Tayyeb Bahrami
tayyeb_bahrami@yahoo.com
5
Ali Zekri
azekri87@gmail.com
6
Mohammad Reza Noori-Daloii
nooridaloii@tums.ac.ir
7
Department of Clinical biochemistry, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran.
Department of Genetics and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
Department of Medical Genetics, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
Department of Medical Genetics, School of Medicine, International Campus, Tehran University of Medical Sciences, Tehran, Iran.
Department of Medical Genetics, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
Department of Medical Genetics and Molecular Biology, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran.
Department of Medical Genetics, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
Waardenburg syndrome (WS) is a neurocristopathy with an autosomal dominant mode of inheritance, and considerable clinical and genetic heterogeneity. WS type II is the most common type of WS in many populations presenting with sensorineural hearing impairment, heterochromia iridis, hypoplastic blue eye, and pigmentary abnormalities of the hair and skin. To date, mutations of MITF, SOX10, and SNAI2 have been implicated in the pathogenesis of WS2. Although different pathogenic mutations have been reported in many ethnic groups, the data on Iranian WS2 patients is insufficient. 31 WS2 patients, including 22 men and 9 women from 14 families were included. Waardenburg consortium guidelines were employed for WS2 diagnosis. WS2 patients underwent screening for MITF, SOX10, and SNAI2 mutations using direct sequencing and MLPA analysis. Clinical evaluation revealed prominent phenotypic variability in Iranian WS2 patients. Sensorineural hearing impairment and heterochromia iridis were the most common features (67% and 45%, respectively), whereas anosmia was the least frequent phenotype. Molecular analysis revealed a de novo heterozygous c.640C>T (p.R214X) in MITF and a de novo heterozygous SOX10 gross deletion in the study population. Our data help illuminate the phenotypic and genotypic spectrum of WS2 in an Iranian series of patients, and could have implications for the genetic counseling of WS in Iran.
http://ijmcmed.org/article-1-798-en.pdf
Waardenburg syndrome type 2
Iran
MLPA
gene deletion
mutation
eng
Babol University of Medical Sciences
International Journal of Molecular and Cellular Medicine (IJMCM)
2251-9637
2251-9645
2018-02
7
1
24
31
article
Overexpression of MiR-138 Inhibits Cell Growth and Induces Caspase-mediated Apoptosis in Acute Promyelocytic Leukemia Cell Line
Rima Manafi shabestari
manafirima@gmail.com
1
Fatemeh Alikarami
fatemehalikarami43@gmail.com
2
Davood Bashash
d.bashash@sbmu.ac.ir
3
Mostafa Paridar
mstparidar@gmail.com
4
Majid Safa
safa.m@iums.ac.ir
5
Department of Hematology and Blood Banking, Faculty of Allied Medicine, Iran University of Medical Sciences, Tehran, Iran.
Department of Hematology and Blood Banking, Faculty of Allied Medicine, Iran University of Medical Sciences, Tehran, Iran.
Department of Hematology and Blood Banking, School of Allied Medical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Ministry of Health and Medical Education, Deputy of Management and Resources Development, Tehran, Iran.
Department of Hematology and Blood Banking, Faculty of Allied Medicine, Iran University of Medical Sciences, Tehran, Iran.
Dysregulated expression of miRNAs can play a vital role in pathogenesis of leukemia. The shortened telomere length, and elevated telomerase activity in acute promyelocytic leukemia cells are mainly indicative of extensive proliferative activity. This study aimed to investigate the effect of overexpression of miR-138 on telomerase activity, and cell proliferation of acute promyelocytic leukemia NB4 cells. MiR-138 was overexpressed in NB4 cells using GFP hsa-miR-138-expressing lentiviruses. hTERT mRNA and protein expression levels were assessed by qRT-PCR and western blot analysis. For evaluation of apoptosis, annexin-V staining and activation of caspases were assessed using flow cytometry and western blot analysis, respectively. Our data demonstrate that overexpression of miR-138 attenuated the hTERT mRNA and protein expression levels. In addition, cell growth was inhibited, and malignant cells underwent caspase mediated-apoptosis in response to miR-138 overexpression. These findings suggest that loss of miR-138 expression may be associated with increased telomerase activity in NB4 cells. Therefore, strategies for up-regulation of miR-138 may result in inhibition of malignant cell growth, and provide a promising therapeutic approach for acute promyelocytic leukemia.
http://ijmcmed.org/article-1-806-en.pdf
Apoptosis
caspase
hTERT
miR-138
poly ADP ribose polymerase (PARP)
eng
Babol University of Medical Sciences
International Journal of Molecular and Cellular Medicine (IJMCM)
2251-9637
2251-9645
2018-02
7
1
32
43
article
Epidemiology of Infantile Visceral Leishmaniasis in Western Algerian And The Convenience of Serum For The Disease Diagnosis by PCR and Immunochromatography
Touria Hadj-Slimane
hadjslimanetouria@gmail.com
1
kheira Senouci
kheirasenouci@yahoo.fr
2
Nori Midoun
semepehuo@gmail.com
3
Assia Bouchetara
bouchetaraa@gmail.com
4
Amel Laradj
ameldj81@yahoo.fr
5
Fadi Bittar
fadi.BITTAR@univ-amu.fr
6
Natural and Life Sciences Faculty, Department of Biology, University of Oran 1 Ahmed Ben Bella, Oran, Algeria.
Natural and Life Sciences Faculty, Department of Biology, University of Oran 1 Ahmed Ben Bella, Oran, Algeria.
Department of Epidemiology and Preventive Medicine, University Hospital of Oran (EHU), Oran, Algeria.
Infectious Diseases Department, Pediatric Hospital of Oran (EHS), Oran, Algeria.
Infectious Diseases Department, Pediatric Hospital of Oran (EHS), Oran, Algeria.
Aix-Marseille University- Faculty of Pharmacy, IHU-Méditerranée Infection, Marseille, France.
Epidemiological situation of infantile visceral leishmaniasis (IVL), which is a public health problem in Algeria, is almost unknown in the cities of Western part of the country. The aim of this study was to analyze the epidemiological, clinical, biological, therapeutic, and evolutionary aspects of IVL in Western Algeria, to evaluate the performance of the immunochromatography as a rapid diagnostic test of the disease, and to propose a diagnosis approach by real-time polymerase chain reaction (RT-PCR) assay from the serum. This prospective study was performed on 63 suspicious cases of visceral leishmaniasis collected from the infectious diseases department at the Pediatric Hospital of Oran from January 2012 to July 2017. For each patient, the epidemiological parameters, and the clinical and biological data were collected. Bone marrow and blood samples were drawn from all cases. Bone marrow was performed to research amastigote forms of Leishmania and to identify the species by PCR-sequencing. Blood samples were used to detect anti-Leishmania antibodies as well as parasite DNA. Patients from the Western regions were mostly from rural areas. Sensitivity of RT-PCR from the bone marrow and from serum was 95.45% and 94.44%, respectively. The immunochromatography allowed the disease’s diagnosis for 11 cases whose myelogram did not confirm the presence of the amastigote forms of Leishmania. Immunochromatography was revealed to be a good technique for disease diagnosis regarding the strongly evocative clinical signs. The results also suggest the interest of the RT-PCR assay from patient serum as a non-invasive sample, in the detection of parasite DNA.
http://ijmcmed.org/article-1-776-en.pdf
Imunochromatography
RT-PCR
bone marrow
serum
sequencing
Leishmania infantum
eng
Babol University of Medical Sciences
International Journal of Molecular and Cellular Medicine (IJMCM)
2251-9637
2251-9645
2018-02
7
1
44
52
article
Marine Actinomycetes with Probiotic Potential and Bioactivity Against Multidrug-resistant Bacteria
Hamed Norouzi
hamed.nt68@yahoo.com
1
Abolghasem Danesh
danesha@mums.ac.ir
2
Mojtaba Mohseni
m.mohseni@umz.ac.ir
3
Mohammad Rabbani khorasgani
m.rabbani@biol.ui.ac.ir
4
Department of Biology, University of Isfahan, Isfahan, Iran.
Biotechnology Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran.
Department of Microbiology, University of Mazandaran, Babolsar, Iran.
Department of Biology, University of Isfahan, Isfahan, Iran.
Considering antimicrobial resistance problem, marine microorganisms with the bioactivity against multi-drug resistant (MDR) pathogens have attracted many scientific interests. To address this issue, a total of 21 marine actinomycetes isolated from the Caspian Sea have been screened out. Primary screening via cross-streak method revealed that 3 strains: MN2, MN39, and MN40 produce antimicrobial agents with wide spectrum activity. In the second step, the potent strains were characterized morphologically, and then identified genetically using 16S rRNA analysis. After that, the bioactivity of the ethyl acetate extracts of liquid culture against some MDR bacteria has been studied using disc diffusion method. Finally, the exoenzymatic activity of the strains, and the anti-vibrio activity of the extracts have been evaluated. The nucleotide sequence of the 16S rRNA gene (1.5 kb) showed that the potent strains belong to the genus Streptomyces. The results of disk diffusion method indicated that among the 3 potent isolates, MN39 and MN2 produce biomolecules with antibacterial activity against MDR bacteria specially methicillin-resistant Staphylococcus aureus (MRSA) and vancomycin-resistant Enterococcus (VRE). In addition, potent strains showed remarkable anti-vibrio activity as well as extracellular enzyme production including amylase and protease. The results of this study revealed that the marine actinomycetes isolated from the sediments of Caspian Sea produce biomolecules effective against MDR bacteria, and suggested that these strains deserve to be studied as potential probiotics due to their anti-vibrio activity besides exoenzyme production.
http://ijmcmed.org/article-1-794-en.pdf
Marine actinomycetes
antimicrobial activity
Caspian Sea
multi-drug resistant bacteria
antivibrio
eng
Babol University of Medical Sciences
International Journal of Molecular and Cellular Medicine (IJMCM)
2251-9637
2251-9645
2018-02
7
1
53
68
article
In silico Homology Modeling and Epitope Prediction of NadA as a Potential Vaccine Candidate in Neisseria meningitidis
Narjes Shahsavani
narjesshahsavani@gmail.com
1
Mohammad Hasan Sheikhha
sheikhha@ssu.ac.ir
2
Hasan Yousefi
Hassanmbi@yahoo.com
3
Fatemeh Sefid
sefid@shahed.ac.ir
4
Shahid Sadoughi University of Medical Sciences and Health Services, Yazd, Iran.
Shahid Sadoughi University of Medical Sciences and Health Services, Yazd, Iran.
Department of Medical Genetics, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
Department of Biology, Science and Art University, Yazd, Iran.
Neisseria meningitidis is a facultative pathogen bacterium which is well founded with a number of adhesion molecules to facilitate its colonization in human nasopharynx track. Neisseria meningitidis is a major cause of mortality from sever meningococcal disease and septicemia. The Neisseria meningitidis adhesion, NadA, is a trimeric autotransporter adhesion molecule which is involved in cell adhesion, invasion, and antibody induction. It is identified in approximately 50% of N. meningitidis isolates, and is established as a vaccine candidate due to its antigenic effects. In the present study we exploited bioinformatics tools to better understand and determine the 3D structure of NadA and its functional residues to select B cell epitopes, and provide information for elucidating the biological function and vaccine efficacy of NadA. Therefore, this study provided essential data to close gaps existing in biological areas. The most appropriate model of NadA was designed by SWISS MODEL software and important residues were determined using the subsequent epitope mapping procedures. Locations of important linear and conformational epitopes were determined and conserved residues were identified to broaden our knowledge of efficient vaccine designing to reduce meningococcal infectious in population. These data now provide a theme to design more broadly cross-protective antigens.
http://ijmcmed.org/article-1-708-en.pdf
Neisseria meningitidis
NadA
epitope prediction
3D structure