eng
Babol University of Medical Sciences
International Journal of Molecular and Cellular Medicine (IJMCM)
2251-9637
2251-9645
2015-07
4
3
143
151
article
Anti-CD19 Monoclonal Antibodies: a New Approach to Lymphoma Therapy
Fatemeh Naddafi
fatemeh.naddafi@sbmu.ac.ir
1
Fatemeh Davami
f_davami@pasteur.ac.ir
2
Pharmaceutical Sciences Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran.
CD19 is expressed on B- lineage cells and follicular dendritic cells and plays a key role in B cell malignancies and autoimmune diseases. Thus, it has been considered as potential target for several monoclonal antibodies (mAbs). For decades, chemotherapy has been known as one of the major antitumor therapies eradicating high proliferative tumor cells. But, anti- CD19 mAbs developed for treating CD19- positive lymphomas and autoimmune diseases would rank among the most novel area of research and development in the pharmaceutical industry. Moreover, several anti- CD19 mAbs are currently being tested in various clinical trials and this review provides an overview of the research accomplished so far.
http://ijmcmed.org/article-1-306-en.pdf
Anti-CD19
monoclonal antibodies
B-cell lymphoma
anti-CD19 sFv
blinatumumab
eng
Babol University of Medical Sciences
International Journal of Molecular and Cellular Medicine (IJMCM)
2251-9637
2251-9645
2015-07
4
3
152
159
article
Study on KAL1 Gene Mutations in Idiopathic Hypogonadotropic Hypogonadism Patients with X-Linked Recessive Inheritance
Atefeh Ahmadzadeh
1
Elahe Ghods
2
Majid Mojarrad
3
Robab Aboutorabi
4
Mojgan Afkhamizadeh
5
Shokoofeh Bonakdaran
6
Zohreh Mosavi
7
Seyed Morteza Taghavi
8
Mohammad Hassanzadeh Nazarabadi
NazarabadiM@mums.ac.ir
9
Department of Medical Genetics, Mashhad University of Medical Sciences, Mashhad, Iran.
Department of Medical Genetics, Mashhad University of Medical Sciences, Mashhad, Iran.
Department of Medical Genetics, Medical School, Mashhad University of Medical Sciences, Mashhad, Iran.
Department of Endocrinology Ghaem Hospital, Mashhad University of Medical Sciences, Mashhad, Iran.
Department of Endocrinology Ghaem Hospital, Mashhad University of Medical Sciences, Mashhad, Iran.
Department of Endocrinology Ghaem Hospital, Mashhad University of Medical Sciences, Mashhad, Iran.
Department of Endocrinology Ghaem Hospital, Mashhad University of Medical Sciences, Mashhad, Iran.
Department of Endocrinology Ghaem Hospital, Mashhad University of Medical Sciences, Mashhad, Iran.
Department of Medical Genetics, Medical School, Mashhad University of Medical Sciences, Mashhad, Iran.
Idiopathic hypogonadotropic hypogonadism (IHH) is a condition caused by low doses of hypothalamic gonadotropin-releasing hormone (GnRH) leading to absence or incomplete sexual maturation. One of the disorders leading to IHH is Kallmann syndrome which is characterized by GnRH deficiency with anosmia or hyposmia. This disorder generally occurs as a hereditary syndrome with X-linked recessive inheritance pattern. However, autosomal dominant or recessive and sporadic cases have also been reported. KAL1 is the most common mutated gene among these patients. The aim of this study was to determine the mutation spectrum of KAL1 gene in twenty patients. KAL1 exons were amplified by PCR technique and the products were assessed by high resolution melting (HRM) technique. In addition, for one of the patients, all coding exons of the KAL1 gene were sequenced. Deletion of exons 4, 5 and 6 were evident in 5%, 10%, and 10% of patients, respectively. Furthermore, HRM results showed hemizygous mutation of exon 12 with more than 95% probability in 25% of patients. Finding these mutations could be helpful in the early diagnosis and presymptomic treatment of Kallman syndrome.
http://ijmcmed.org/article-1-291-en.pdf
Idiopathic hypogonadotropic hypogonadism
kallmann syndrome
KAL1 gene
X-linked recessive
GnRH
eng
Babol University of Medical Sciences
International Journal of Molecular and Cellular Medicine (IJMCM)
2251-9637
2251-9645
2015-07
4
3
160
166
article
Analysis of rs6725887 in the WD Repeat Protein 12 in Association With Coronary Artery Disease in Iranian Patients
Mohammad piryaei
m.piryaei@gmail.com
1
Sayyed Mohammad Hossein Ghaderian
sghaderian@yahoo.co.uk
2
Hossein Vakili
hvakili@yahoo.com
3
Hooshang Zaimkohan
dr.zaim@gmail.com
4
Nastaran Mohammadi Ghahhari
molecularmedicine67@gmail.com
5
Maryam Mafi Golchin
maryam.mafi@rocketmail.com
6
Department of Medical Genetics, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Department of Medical Genetics, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Cardiovascular Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Department of Medical Genetics, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
Department of Biochemistry, Pasteur Institute of Iran, Tehran, Iran.
Department of Genetics and Anatomy, Babol University of Medical Sciences, Babol, Iran.
Although genetic variants that affect susceptibility to coronary artery disease (CAD) have been greatly known, a number of these single nucleotide polymorphisms (SNPs) remain to be analyzed in populations with different ethnicities. CAD is influenced by numerous genetic, environmental, and lifestyle factors, and is an important reason for mortality around the globe. In this study, a novel SNP (rs6725887) in the WD Repeat Protein 12 (WDR12) gene was selected to be examined in Iranian patients with CAD. Ninety eigth healthy controls and one hundred and one CAD patients were enrolled from Iranian population, and their clinical data were collected for further comparisons. After DNA extraction from each sample, genotypes were characterized by Taq Man probe real- time PCR assay. Statistical analyses were performed to evaluate genotype and allele frequencies and compared the values with clinical variables. Body mass index, blood pressure, fasting blood sugar, LDL, HDL, cholesterol, and triglyceride significantly differed in CAD and control groups. Genotype and allele frequencies of rs6725887 in CAD patients and controls showed no significant association in the distribution. However, clinical parameters of CAD patients like HDL, LDL, FBS, TG, DBP and SBP had significantly (P<0.05) higher levels compared to control group. The rs6725887 polymorphism is unlikely to play a key role in CAD risk in our population. Further additional samples are required for better appreciation of the influence of WDR12 SNP on CAD occurrence.
http://ijmcmed.org/article-1-341-en.pdf
Atherosclerosis
WDR12 gene
polymorphism
risk factor
Iranian
eng
Babol University of Medical Sciences
International Journal of Molecular and Cellular Medicine (IJMCM)
2251-9637
2251-9645
2015-07
4
3
167
173
article
The Frequency of Exotoxin A and Exoenzymes S and U Genes Among Clinical Isolates of Pseudomonas aeruginosa in Shiraz, Iran
Arshid Yousefi-Avarvand
arshid.yousefi5@gmail.com
1
Reza Khashei
khasheir@sums.ac.ir
2
Hadi Sedigh Ebrahim-Saraie
seddigh.hadi@gmail.com
3
Amir Emami
vahid.s13@gmail.com
4
Kamiar Zomorodian
zomorodian@sums.ac.ir
5
Mohammad Motamedifar
motamedm@sums.ac.ir
6
Department of Bacteriology and Virology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
Department of Bacteriology and Virology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
Department of Bacteriology and Virology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
Department of Bacteriology and Virology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
Department of Parasitology and Mycology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
Department of Bacteriology and Virology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
Pseudomonas aeruginosa as an opportunistic pathogen produces several virulence factors. The most important of these factors are exotoxin A and type III secretion system (T3SS). The aim of this study was to determine the frequency of toxA, exoU and exoS genes among clinical isolates of P. aeruginosa. In this cross-sectional study from September 2011 to February 2012, 156 P. aeruginosa isolates were recovered from different clinical samples. Susceptibility testing against 10 antibiotics was performed on individual isolates by the disc diffusion method according to CLSI guidelines. Extracted DNA was subjected to PCR assay for determining the presence of toxA, exoU and exoS genes. Overall, the frequency of toxA, exoU and exoS genes were 90.4%, 66.7% and 65.4%, respectively. All of the abdominal and eye isolates were exoS+. The frequency of exoS+/exoU- and exoS-/exoU+ genotypes was estimated 19.2% and 16.2%, respectively. Indeed, genotypes exoS+/exoU+ and exoS-/exoU- were found with frequencies of 48.7% and 15.3%, respectively. The highest and lowest antibiotic resistance rate was seen against azteroenam (94.2%) and amikacin (44.9%), respectively. Fluoroqinolone-resistant isolates were isolated with frequency of 45.8%. Multi-drug resistant (MDR) isolates were detected in 62.8% of isolates. The resistance rate in exoU+ isolates was 86% compared to 66% in exoS+ isolates. The high frequencies of virulence genes detected in our clinical isolates with notable antibiotic resistance rates indicate the potential risk of these isolates in nosocomial infections.
http://ijmcmed.org/article-1-337-en.pdf
Pseudomonas aeruginosa
exotoxin A
exoenzyme S
exoenzyme U
eng
Babol University of Medical Sciences
International Journal of Molecular and Cellular Medicine (IJMCM)
2251-9637
2251-9645
2015-07
4
3
174
181
article
Diversity of Variable Number Tandem Repeat Loci in Shigella Species Isolated From Pediatric Patients
Reza Ranjabr
ranjbarre@gmail.com
1
Mojtaba Memariani
memaryani@gmail.com
2
Hamed Memariani
h.memariani@gmail.com
3
Molecular Biology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran.
Molecular Biology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran.
Department of Molecular and Cell Biology, Faculty of Basic Sciences, University of Mazandaran, Babolsar, Iran.
Multilocus variable number tandem repeat (VNTR) analysis (MLVA) is a new typing method with several advantages compared to other methods. Dissemination of Shigella is highly significant in developing countries. Whilst Shigella is becoming increasingly important as an etiologic agent of pediatric shigellosis in Iran, little is known about the genetic diversity of the local strains. Therefore, the aim of this study was to describe the genetic diversity of Shigella species isolated from pediatric patients in Tehran, Iran. A total of 53 Shigella isolates were obtained from 1070 patients with diarrhea (less than 12 years of age). All isolates were identified by routine biochemical and serological tests. The confirmed Shigella isolates were further serogrouped (by the slide agglutination) using slide agglutination method. MLVA assay with the seven loci resolved 53 Shigella isolates into 36 different genotypes. Almost all the isolates were classified into five clonal complexes. Furthermore, our MLVA assay could effectively distinguish the four Shigella species. This study has provided valuable insights into the genetic heterogeneity of Shigella species in Tehran, Iran. Our findings can be helpful for further epidemiological surveillance of Shigella species in this country in the future.
http://ijmcmed.org/article-1-311-en.pdf
Shigella
MLVA
genotyping
eng
Babol University of Medical Sciences
International Journal of Molecular and Cellular Medicine (IJMCM)
2251-9637
2251-9645
2015-07
4
3
182
187
article
Expression of Melanocortin 4 Receptor mRNA in Male Rat Hypothalamus During Chronic Stress
Maryam Karami Kheirabad
marya.karami@gmail.com
1
Bahia Namavar Jahromi
namavarb@sums.ac.ir
2
Amin Tamadon
amintamaddon@yahoo.com
3
Amin Ramezani
amin.ramezani@gmail.com
4
Somayeh Ahmadloo
parisahmadloo2014@gmail.com
5
Fatemeh Sabet Sarvestani
dfssarvestani@yahoo.com
6
Omid Koohi-Hosseinabadi
koohiomid@yahoo.com
7
Department of Basic Sciences, Azad University, Gachsaran Branch, Gachsaran, Iran.
Infertility Research Center, Department of OB-GYN, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
Transgenic Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
Department of Medical Biotechnology, School of Advanced Medical Sciences and Technology, Shiraz University of Medical Sciences, Shiraz, Iran.
Infertility Research Center, Department of OB-GYN, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
Infertility Research Center, Department of OB-GYN, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
Laboratory Animal Center, Shiraz University of Medical Sciences, Shiraz, Iran.
The effects of chronic stress and glucocorticoids receptor antagonist (RU486) on expression of melanocortin 4 receptor (MC4R) mRNA in arcuate nucleus (ARC) of male rats were evaluated. In this study, adult male Sprague Dawley rats were placed into four groups (n=6/group) stress, RU486, stress/RU486, and control groups. In stress group, the rats were restrained, 1 h/day, for 12 days. In RU486 group, the rats were injected RU486 for 12 days. In stress/RU486 group, the rats were injected RU486 1 h before the stress process for 12 days. Relative expression of MC4R mRNA was determined using real-time PCR. Relative expression of MC4R mRNA in the stress group was higher than that of the control rats (P0.05). Chronic restraint stress causes increase in mRNA expression of MC4R in ARC and blockade of glucocorticoid receptors has no effect on this up-regulation.
http://ijmcmed.org/article-1-336-en.pdf
Chronic stress
melanocortin 4 receptor (MC4R)
hypothalamus
rats
eng
Babol University of Medical Sciences
International Journal of Molecular and Cellular Medicine (IJMCM)
2251-9637
2251-9645
2015-07
4
3
188
195
article
Impact of Propionic Acid on Liver Damage in Rats
Sooad Al- daihan
SDAIHAN@ksu.edu.sa
1
Ramesa Shafi Bhat
rbhat@ksu.edu.sa
2
Cellular Biochemistry Department, Science College, King Saud University, Riyadh, Saudi Arabia.
Cellular Biochemistry Department, Science College, King Saud University, Riyadh, Saudi Arabia.
Propionic acid (PA) is a short chain fatty acid, a common food preservative and metabolic end product of enteric bacteria in the gut. The present study was undertaken to investigate the effect of PA on liver injury in male rats. Male western albino rats were divided into two groups. The first group served as normal control, the second was treated with PA. The activities of serum hepatospecific markers such as aspartate transaminase, alanine transaminase, and alkaline phosphatase were estimated. Antioxidant status in liver tissues was estimated by determining the level of lipid peroxidation and activities of enzymatic and non-enzymatic antioxidants. Sodium and potassium levels were also measured in liver tissue. PA treatment caused significant changes in all hepatospecific markers. Biochemical analysis of liver homogenates from PA-treated rats showed an increase in oxidative stress markers like lipid peroxidation and lactate dehydrogenase, coupled with a decrease in glutathione, vitamin C and glutathione S- transferase. However, PA exposure caused no change in sodium and potassium levels in liver tissue. Our study demonstrated that PA persuade hepatic damage in rats.
http://ijmcmed.org/article-1-293-en.pdf
Propionic acid
liver
oxidative stress
eng
Babol University of Medical Sciences
International Journal of Molecular and Cellular Medicine (IJMCM)
2251-9637
2251-9645
2015-07
4
3
196
198
article
Requirement of a Blocking Step in Affinity Purification of Polyclonal Antibodies
Sepideh Hamzehlou
s-hamzehlou@razi.tums.ac.ir
1
Paul R. Albert
palbert@uottawa.ca
2
Mohammad M Farajollahi
fam-biotec@iums.ac.ir
3
Cellular and Molecular Research Center, Department of Medical Biotechnology, Faculty of Allied Medical Sciences, Iran University of Medical Sciences, Tehran, Iran.
Ottawa Hospital Research Institute, Department of Medicine, Faculty of Medicine, University of Ottawa, Ottawa, Canada.
Cellular and Molecular Research Center, Department of Medical Biotechnology, Faculty of Allied Medical Sciences, Iran University of Medical Sciences, Tehran, Iran.
http://ijmcmed.org/article-1-359-en.pdf
Non-specific binding
antibody purification
Blocking