International Journal of Molecular and Cellular Medicine
مجله بین المللی سلولی و مولکولی
Int J Mol Cell Med
Medical Sciences
http://ijmcmed.org
1
admin
2251-9637
2251-9645
10.22088/IJMCM.BUMS
en
jalali
1392
5
1
gregorian
2013
8
1
2
3
online
1
fulltext
en
Sarcocystis cruzi: First molecular identification from cattle in Iran
Other
Other
Original Article
Original Article
Sarcocystis is a genus of cyst-forming parasites infecting both animals and human. This study aimed to isolate coccidian tissue cysts from muscle of infected animals by a simple method in addition to molecular identification of Sarcocystis cruzi from the samples. The samples were obtained from commercial source in Babol, Northern Iran. Five grams of calf muscle was cut into 2-3 pieces in 30 ml of phosphate-buffered saline containing 0.1% Tween 80 and homogenized with IKA T25, DIGITAL ULTRA-TURRAX. The homogenate was filtrated twice and used for microscopy examination and molecular analysis. Polymerase chain reaction (PCR) and partial sequence analysis of the 18S ribosomal gene were used to identify the Sarcocystis species. Giemsa stain of the filtrated calf muscle samples showed that the sample had ellipse to around tissue cysts contained crescent-shaped bodies. The PCR of the 18SrDNA yielded an 1100 bp DNA band on agarose gel and sequence analysis of the DNA confirmed the isolate as S. cruzi. The sequence was deposited in GenBank by Accession No.KC508514. This is the first molecular identification of an isolate of S. cruzi in Iran.
Cattle, iran, Sarcocystis cruzi, apicomplexa
125
130
http://ijmcmed.org/browse.php?a_code=A-10-51-1&slc_lang=en&sid=1
Narges
Kalantari
nfkala@yahoo.com
10031947532846003608
10031947532846003608
No
Cellular and Molecular Biology Research Center (CMBRC), Babol University of Medical Sciences, Babol, Iran.
Masomeh
Bayani
m_baiany@yahoo.com
10031947532846003609
10031947532846003609
No
Infectious Diseases and Tropical Medicine Research Center, Babol University of Medical Sciences, Babol, Iran.
Salaman
Ghaffari
s3ghaffari@yahoo.com
10031947532846003610
10031947532846003610
Yes
Department of Parasitology–Mycology, Faculty of Medicine, Babol University of Medical Sciences, Babol, Iran.