International Journal of Molecular and Cellular Medicine
مجله بین المللی سلولی و مولکولی
Int J Mol Cell Med
Medical Sciences
http://ijmcmed.org
1
admin
2251-9637
2251-9645
10.22088/IJMCM.BUMS
en
jalali
1392
3
1
gregorian
2013
6
1
2
2
online
1
fulltext
en
The Assessment of Early Glycosaminoglycan Concentration Changes in the Kidney of Diabetic Rats by Critical Electrolyte Concentration Staining
Clinical Biochemistry
Clinical Biochemistry
Original Article
Original Article
Glycosaminoglycan (GAG) has a pivot role in renal function and homeostasis. Analysis of GAG amount generally serves to determine GAG alteration due to diabetes mellitus. Critical Electrolyte Concentration (CEC) staining can be an efficacy method to study GAG amount changes. Based on an experimental study, 20 male rats were randomly divided equally into two experimental and control groups. Diabetes mellitus was induced by a single sub cutaneous injection (120 mg/kg) of alloxan monohydrate. After 8 weeks, diabetic kidneys were paraffin embedded and sectioned at 5μm on a microtome. Slides were prepared and studied after staining by Critical Electrolyte Concentration (CEC 1 -4). In this study, we succeeded to show a decrease of hyaluronic acid and chondroitin sulfate concentration in diabetic kidney at 8 weeks diabetic rats which are earlier signs compared to those reported previously. In contrary, no significant changes in heparin sulfate and keratin sulfate have been seen. Diabetic nephropathy is a progressive disease and earlier diagnosis makes a better treatment design to reduce its development. CEC staining is able to determine degradation of hyaluronic acid and chondroitin sulfate synthesis in diabetic kidney of rats in an earlier time.
Glycosaminoglycan, Diabetes mellitus, kidney, CEC
58
63
http://ijmcmed.org/browse.php?a_code=A-10-63-3&slc_lang=en&sid=1
Mohsen
Pourghasem
mpourghasem@hotmail.com
10031947532846003634
10031947532846003634
No
Cellular and Molecular Biology Research Center (CMBRC), Babol University of Medical Sciences, Babol, Iran.
Ebrahim
Nasiri
10031947532846003635
10031947532846003635
No
Department of anatomical Sciences, GeilanUniversity of Medical Sciences, Rasht, Iran.
Shima
Sum
10031947532846003636
10031947532846003636
No
Department of Social Medicin, Babol University of Medical Sciences, Babol, Iran.
Hamid
Shafi
dr_hamidshafi@yahoo.com
10031947532846003637
10031947532846003637
Yes
Department of Urology, Babol University of Medical Sciences, Babol, Iran.