International Journal of Molecular and Cellular Medicine
مجله بین المللی سلولی و مولکولی
Int J Mol Cell Med
Medical Sciences
http://ijmcmed.org
1
admin
2251-9637
2251-9645
10.22088/IJMCM.BUMS
en
jalali
1396
11
1
gregorian
2018
2
1
7
1
online
1
fulltext
en
Epidemiology of Infantile Visceral Leishmaniasis in Western Algerian And The Convenience of Serum For The Disease Diagnosis by PCR and Immunochromatography
Infectious disease (Molecular and Cellular aspects)
Infectious disease (Molecular and Cellular aspects)
Original Article
Original Article
<div style="text-align: justify;">Epidemiological situation of infantile visceral leishmaniasis (IVL), which is a public health problem in Algeria, is almost unknown in the cities of Western part of the country. The aim of this study was to analyze the epidemiological, clinical, biological, therapeutic, and evolutionary aspects of IVL in Western Algeria, to evaluate the performance of the immunochromatography as a rapid diagnostic test of the disease, and to propose a diagnosis approach by real-time polymerase chain reaction (RT-PCR) assay from the serum. This prospective study was performed on 63 suspicious cases of visceral leishmaniasis collected from the infectious diseases department at the Pediatric Hospital of Oran from January 2012 to July 2017. For each patient, the epidemiological parameters, and the clinical and biological data were collected. Bone marrow and blood samples were drawn from all cases. Bone marrow was performed to research amastigote forms of <em>Leishmania </em>and to identify the species by PCR-sequencing. Blood samples were used to detect anti-<em>Leishmania</em> antibodies as well as parasite DNA. Patients from the Western regions were mostly from rural areas. Sensitivity of RT-PCR from the bone marrow and from serum was 95.45% and 94.44%, respectively. The immunochromatography allowed the disease’s diagnosis for 11 cases whose myelogram did not confirm the presence of the amastigote forms of <em>Leishmania</em>. Immunochromatography was revealed to be a good technique for disease diagnosis regarding the strongly evocative clinical signs. The results also suggest the interest of the RT-PCR assay from patient serum as a non-invasive sample, in the detection of parasite DNA.</div>
Imunochromatography, RT-PCR, bone marrow, serum, sequencing, Leishmania infantum
32
43
http://ijmcmed.org/browse.php?a_code=A-10-1311-1&slc_lang=en&sid=1
Touria
Hadj-Slimane
hadjslimanetouria@gmail.com
100319475328460011393
100319475328460011393
Yes
Natural and Life Sciences Faculty, Department of Biology, University of Oran 1 Ahmed Ben Bella, Oran, Algeria.
kheira
Senouci
kheirasenouci@yahoo.fr
100319475328460011394
100319475328460011394
No
Natural and Life Sciences Faculty, Department of Biology, University of Oran 1 Ahmed Ben Bella, Oran, Algeria.
Nori
Midoun
semepehuo@gmail.com
100319475328460011395
100319475328460011395
No
Department of Epidemiology and Preventive Medicine, University Hospital of Oran (EHU), Oran, Algeria.
Assia
Bouchetara
bouchetaraa@gmail.com
100319475328460011396
100319475328460011396
No
Infectious Diseases Department, Pediatric Hospital of Oran (EHS), Oran, Algeria.
Amel
Laradj
ameldj81@yahoo.fr
100319475328460011397
100319475328460011397
No
Infectious Diseases Department, Pediatric Hospital of Oran (EHS), Oran, Algeria.
Fadi
Bittar
fadi.BITTAR@univ-amu.fr
100319475328460011398
100319475328460011398
No
Aix-Marseille University- Faculty of Pharmacy, IHU-Méditerranée Infection, Marseille, France.