International Journal of Molecular and Cellular Medicine
مجله بین المللی سلولی و مولکولی
Int J Mol Cell Med
Medical Sciences
http://ijmcmed.org
1
admin
2251-9637
2251-9645
10.22088/IJMCM.BUMS
en
jalali
1396
8
1
gregorian
2017
11
1
6
4
online
1
fulltext
en
Association of CpG-SNP and 3'UTR-SNP of WFS1 with the Risk of Type 2 Diabetes Mellitus in an Iranian Population
Genetics & Disease
Genetics & Disease
Original Article
Original Article
<div style="text-align: justify;">Type 2 diabetes mellitus (T2DM) is one of the most common multifactorial disorders in Iran. Recent genome wide association studies (GWASs), and functional studies have suggested that <em>WFS1</em> may predispose individuals to T2DM. However, to date, the possible association of such variants with T2DM in Iranians remained unknown. Here, we investigated the association of the two polymorphisms of <em>WFS1</em> (rs1801214 a CpG-SNP, and rs1046320 a 3’UTR-SNP) with T2DM in an Iranian population. The study population comprised 432 unrelated Iranian individuals including 220 patients with T2DM, and 211 unrelated healthy control subjects. Genotyping was performed using PCR-RFLP, and confirmed with sequencing. In a logistic regression analysis, the rs1801214-T allele was associated with a significantly lower risk of T2DM assuming the log-additive model (OR: 0.68, 95% CI: 0.52-0.91, P= 0.007539). Moreover, the G allele of rs1046320 was associated with a lower risk of T2DM assuming the log-additive model (OR: 0.68, 95% CI: 0.50- 0.91, P= 0.008313). Haplotype analysis revealed that haplotypes that carry at least one protective allele are associated with a lower risk of T2DM. This is a first evidence for the association of<em> WFS1</em> rs1801214, and rs1046320 with T2DM in an Iranian population.</div>
WFS-1, type 2 diabetes, genome wide association study, single nucleotide polymorphisms
197
203
http://ijmcmed.org/browse.php?a_code=A-10-822-3&slc_lang=en&sid=1
Shahram
Torkamandi
torkamandi.shahram@yahoo.co.uk
100319475328460010748
100319475328460010748
No
Department of Medical Genetics, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Milad
Bastami
mi.bastami@live.com
100319475328460010749
100319475328460010749
No
Department of Medical Genetics, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.
Hamid
Ghaedi
qaedi65@gmail.com
100319475328460010750
100319475328460010750
No
Department of Medical Genetics, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Shahriar
Tarighi
shahryartarighi@sbmu.ac.ir
100319475328460010751
100319475328460010751
No
Department of Medical Laboratory Sciences, School of Allied Medical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Fazlollah
Shokri
shahram.torkamandi@gmail.com
100319475328460010752
100319475328460010752
No
Department of Medical Genetics, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Abdolreza
Javadi
reza.javadi@sbmu.ac.ir
100319475328460010753
100319475328460010753
No
Department of Pathology, Imam Hossein Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Reza
Mirfakhraie
r.mirfakhraie@sbmu.ac.ir
100319475328460010754
100319475328460010754
No
Department of Medical Genetics, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Mir Davood
Omrani
davood_omrani@sbmu.ac.ir
100319475328460010755
100319475328460010755
Yes
Department of Medical Genetics, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.