en Genetics & Disease Genetics & Disease Original Article Original Article <p style="text-align: justify;">Coronary artery disease (CAD) including myocardial infarction (MI) as its complication, is one of the most common heart diseases worldwide and also in Iran, with extremely elevated mortality. CAD is a multifactorial disorder. Twin and family studies at different loci have demonstrated that genetic factors have an important role in the progression of CAD. Many studies have reported a significant association of <em>CDKN2B-AS</em>, also known as <em>ANRIL</em> which is located within the p15, p16, p14 gene cluster at 9p21 locus,&nbsp; with cardiovascular diseases as well as many other diseases like diabetes and cancers. This study investigated two polymorphisms rs10757274 and rs1333042 of <em>CDKN2B-AS</em> gene at 9p21 locus. 205 subjects, comprising 102 controls and 103 CAD patients were genotyped by TaqMan probe real time PCR technique and haplotypes were examined. This study confirmed the association of rs10757274 variants with CAD in Iranian patients (P= 0.003) but genotype and allele distributions of CAD and control groups showed no significant association for the rs1333042. However, frequency of the [G;G] haplotype of these two SNPs was significantly higher in CAD group (P= 0.0002, Odds Ratio = 3.1, 95% CI = 1.7-5.7). Our finding suggests that [G; G]&nbsp; haplotype of rs10757274 and rs1333042 may be considered&nbsp; as a genetic risk factor for susceptibility to CAD in Iranian patients.</p> Single nucleotide polymorphism, risk factor, coronary artery disease, CDKN2B-AS, Iranian 31 37 http://ijmcmed.org/browse.php?a_code=A-10-763-1&slc_lang=en&sid=1 Maryam Mafi Golchin maryam.mafi@rocketmail.com 10031947532846008635 10031947532846008635 No Department of Genetics, Faculty of Medicine, Babol University of Medical Sciences, Babol, Iran. Sayyed Mohammad Hossein Ghaderian sghaderian@yahoo.co.uk 10031947532846008636 10031947532846008636 Yes Department of Medical Genetics, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran. Haleh Akhavan-Niaki halehakhavan@yahoo.com 10031947532846008637 10031947532846008637 No Department of Genetics, Faculty of Medicine, Babol University of Medical Sciences, Babol, Iran. Rozita Jalalian rozita_jalalian@yahoo.com 10031947532846008638 10031947532846008638 No Cardiovascular Research Center, Mazandaran University of Medical Sciences, Sari, Iran. Laleh Hedari khanomeheidari@yahoo.com 10031947532846008639 10031947532846008639 No Department of Medical Genetics, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran. Ali Reza Salami asalami@ut.ac.ir 10031947532846008640 10031947532846008640 No Department of Biotechnology, University of Tehran, Tehran, Iran.