en Stem Cell Stem Cell Original Article Original Article <p style="text-align: left">Mesenchymal stem cells (MSCs) have the ability to differentiate into neuronal like cells under appropriate culture condition. In this study, we investigated whether MSCs derived from human peripheral blood (PB-MSCs) can differentiate into neuronal like cells by synergic effect of the growth factors EGF, bFGF and Noggin. For this purpose, the expression of five neuronal markers (Nestin, &beta III tubulin, <em>NFM</em>, <em>MAP2</em> and <em>NSE</em>) were evaluated in treated PB-MSCs by SYBR Green Real time PCR. The expression analysis showed a higher expression of &beta- tubulin and <em>NFM</em> in treated BP-MSCs compared with untreated PB-MSCs as a control group. The expression of Nestin was also diminished in PB-MSCs treated with Noggin. This study suggested that the treatment of PB- MSCs with Noggin alongside with bFGF and EGF might differentiate these cells into neuronal lineage cells. The obtained results could be further developed for useful applications in regenerative medicine.</p> Mesenchymal Stem Cells, Differentiation, Neuronal markers, Noggin 209 217 http://ijmcmed.org/browse.php?a_code=A-10-534-1&slc_lang=en&sid=1 Zahra Fazeli zahrafazeli6@gmail.com 10031947532846006049 10031947532846006049 No Department of Medical Genetics, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran. Masoumeh Rajabibazl masoumeh_rajabibazl@yahoo.com 10031947532846006050 10031947532846006050 No Department of Medical Genetics, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran. Siamak Salami salami.si@gmail.com 10031947532846006051 10031947532846006051 No Department of Clinical Biochemistry, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran. Nader Vazifeh Shiran nadershirani@yahoo.com 10031947532846006052 10031947532846006052 No Department of Clinical Biochemistry, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran. Sayyed Mohammad Hossein Ghaderian s.ghaderian@yahoo.co.uk 10031947532846006053 10031947532846006053 No Department of Hematology, School of Allied Medical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran. Mir Davood Omrani davood_omrani@yahoo.co.uk 10031947532846006054 10031947532846006054 Yes Department of Medical Genetics, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.