International Journal of Molecular and Cellular Medicine
مجله بین المللی سلولی و مولکولی
Int J Mol Cell Med
Medical Sciences
http://ijmcmed.org
1
admin
2251-9637
2251-9645
10.22088/IJMCM.BUMS
en
jalali
1393
12
1
gregorian
2015
3
1
4
2
online
1
fulltext
en
Virulence Factors Variation Among Bordetella Pertussis Isolates in Iran
Genetics & Disease
Genetics & Disease
Letters to the editor
Letters to the editor
Pertussis is still endemic and the recently resurgence of the disease caused by Bordetella pertussis has been shown in many countries. The polymorphism of the virulence genes of B. pertussis and lack of any information about the allelic variation between the Iranian isolates promotes us to analysis of the genes encoded virulence factors including ptxS1, prn, fim3 and cya to understand the differences between circulating strains and vaccine strains. PCR and DNA sequencing of the genes was performed on 31 strains collected from patients in Iran from 2008-2010 after biochemical detection of the isolates. Sequencing results of prn, ptxS1, fim3 and cya genes indicated only one allele of the genes among the isolates including ptxS1A, prn2, fim3B and cyaA2, respectively. The alleles of the studied genes were different from vaccine strains (ptxS1E and B, prn2 and 6, fim3A and cyaA1 and 2) in Iran. Vaccination allows the spread of escape mutants and selects for the strains having different alleles of the genes from vaccine strains. The strains used for vaccine in our country are the same as some other countries in the world and our results of alleles of the studied genes were also the same. It is postulated that similar trend and schedules in vaccination strategies and similar coverage of vaccination and the same vaccines may lead to similar results from variation in the virulence factors involving in the immune response and variation is not related to geographic era.
Bordetella pertussis, virulence factors, polymorphism, vaccine
138
142
http://ijmcmed.org/browse.php?a_code=A-10-363-1&slc_lang=en&sid=1
Vajiheh Sadat
Nikbin
vsnikbin@yahoo.com
10031947532846004826
10031947532846004826
No
Pertussis Reference Laboratory, Department of Bacteriology, Pasteur Institute of Iran.
Nazanin
Jannesar Ahmadi
just_microbiology@yahoo.com
10031947532846004827
10031947532846004827
No
Pertussis Reference Laboratory, Department of Bacteriology, Pasteur Institute of Iran.
Maryam
Hosseinpour
maryamhoseinpour20@gmail.com
10031947532846004828
10031947532846004828
No
Pertussis Reference Laboratory, Department of Bacteriology, Pasteur Institute of Iran.
Masoumeh
Nakhost Lotfi
naderehl@yahoo.com
10031947532846004829
10031947532846004829
No
Pertussis Reference Laboratory, Department of Bacteriology, Pasteur Institute of Iran.
Fahimeh
Shooraj
fahimeh51@yahoo.com
10031947532846004830
10031947532846004830
No
Pertussis Reference Laboratory, Department of Bacteriology, Pasteur Institute of Iran.
Fatemeh Sadeghpour
Sadeghpour
shahcheraghifereshteh@yahoo.com
10031947532846004831
10031947532846004831
Yes
Pertussis Reference Laboratory, Department of Bacteriology, Pasteur Institute of Iran.
Fereshteh
Shahcheraghi
10031947532846004832
10031947532846004832
No
Pertussis Reference Laboratory, Department of Bacteriology, Pasteur Institute of Iran.