en Nanomedicine Nanomedicine Original Article Original Article <p style="text-align: justify;"><span style="font-size:12px;"><span style="font-family:Tahoma;"><span style="line-height:14.0pt">Gold nanoparticles (AuNPs) are a suitable choice in the biomedical field due to their physical properties. The presence of gold nanoparticles, acting alongside mesenchymal stem cells (MSCs) through paracrine interactions, accelerates wound closure, enhances angiogenesis, favorably regulates extracellular matrix remodeling, and encourages skin regeneration with typical construction and function. In this study, we hypothesized that AuNPs Nano scaffolds would enhance the proliferation and specialization potential of human adipose-derived mesenchymal stem cells (hADSC in the wound area during <i>in vitro</i>, <i>In vivo</i> studies. To evaluate the nanoparticles; transmission electron microscopy was used to detect AuNPs and dynamic light scattering assay was used to estimate their size. Immunofluorescence staining, MTT assay, and semi-quantitative histological analysis were performed. Our results show that AuNPs at non-toxic concentrations (6 ppm) improve collagen content deposition and micro vessels expression induces cell migration and therapeutic efficacy in wound healing by hADSC at 14 and 28 days after surgery. As a result, AuNPs promote differentiation through a paracrine signaling pathway. Therefore, hADSC cultured with AuNPs can be used as a reagent to enhance skin tissue formation.</span></span></span></p> Dynamic Light Scattering, Paracrine Communication, Metal Nanoparticles, Collagen, Fluorescent Transmission, Cell Proliferation, Angiogenesis 1171 1182 http://ijmcmed.org/browse.php?a_code=A-10-8756-1&slc_lang=en&sid=1 Faezeh Salehi Nasab faezeh.salehi227@gmail.com 100319475328460037117 100319475328460037117 No Student Research Committee, Lorestan University of Medical Sciences, Khorramabad, Iran Mehdi Birjandi mehdibirjandi@gmail.com 100319475328460037118 100319475328460037118 No Department of Nutritional Health Research Center, School of Health and Nutrition, Lorestan University of Medical Sciences, Khorramabad, Iran. Bahram Rasoulian bahramrasoulian@gmail.com 100319475328460037119 100319475328460037119 No Department of Physiology and Pharmacology, School of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran. Farzaneh Chehelcheraghi fr.chehelcheraghi@gmail.com 100319475328460037120 100319475328460037120 Yes Gastroenterology and Liver Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran Marzieh Rashidipour m_rashidi80@yahoo.com 100319475328460037121 100319475328460037121 No Nutritional Health Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran.