en Infectious disease (Molecular and Cellular aspects) Infectious disease (Molecular and Cellular aspects) Original Article Original Article <div style="text-align: justify;"><span style="font-size:12px;"><span style="font-family:Tahoma;"><a name="_Hlk184577878"><span style="line-height:107%">Multi-drug-resistant (MDR) <i>Acinetobacter</i> <i>baumannii</i> has become a major global healthcare concern due to its opportunistic infections and high antibiotic resistance. </span></a><span style="line-height:107%">This investigation is intended to investigate curcumin&#39;s potential anti-bacterial and antibiofilm impacts on MDR <i>A</i>. <i>baumannii</i> and to present a promising strategy for fighting against infections caused by this pathogen. This cross-sectional investigation comprised 34 MDR <i>A</i>. <i>baumannii</i> clinical isolates. The Kirby-Bauer disc diffusion method evaluated the sensitivity of isolates to multifaceted anti-bacterial agents. The microdilution broth method quantified curcumin&#39;s minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC). The efficacy of curcumin in inhibiting MDR <i>A</i>. <i>baumannii</i> biofilm was assessed via 96-well microtiter plates. The expression of the biofilm-associated protein (<i>bap</i>) gene was evaluated by employing quantitative real-time PCR (qRT-PCR). Within the 34 MDR <i>A</i>. <i>baumannii</i> isolates, the highest resistance was noted for trimethoprim/sulfamethoxazole and ciprofloxacin, with all 34 isolates (100%) indicating resistance. The lowest resistance was noted for ampicillin/sulbactam, with 22 isolates (64.7%) exhibiting resistance. The MICs of curcumin ranged from 0.625 to 2.5 mg/ml, while the MBCs varied between 1.25 to 5 mg/ml. Curcumin reduced biofilm formation by 25% to 91%, depending on the concentration. In contrast to the untreated control, the average relative activity of the <i>bap</i> gene in MDR <i>A. baumannii</i> isolates declined by 62.07%. The findings indicate that curcumin <a name="_Hlk183516429">demonstrates</a> antimicrobial and anti-biofilm activities against MDR <i>A</i>. <i>baumannii</i>. The downregulation noted in the <i>bap</i> gene further supports the curcumin&#39;s anti-biofilm impact.</span></span></span></div> Acinetobacter baumannii, Multidrug-resistance, Curcumin, Biofilm. 567 575 http://ijmcmed.org/browse.php?a_code=A-10-6818-2&slc_lang=en&sid=1 Kasra Javadi Kasrajavadii@gmail.com 100319475328460033605 0009-0005-1813-9443 No Department of Microbiology, Faculty of Medicine, Shahed University, Tehran, Iran. Mohammad Hossein Ahmadi mhahmadi@shahed.ac.ir 100319475328460033606 100319475328460033606 Yes Department of Microbiology, Faculty of Medicine, Shahed University, Tehran, Iran. Mehdi Rajabnia ramazan69@yahoo.com 100319475328460033607 100319475328460033607 No Infectious Diseases and Tropical Medicine Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran. Mehrdad Halaji mehrdad.md69@gmail.com 100319475328460033608 100319475328460033608 No Infectious Diseases and Tropical Medicine Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran.