International Journal of Molecular and Cellular Medicine
مجله بین المللی سلولی و مولکولی
Int J Mol Cell Med
Medical Sciences
http://ijmcmed.org
1
admin
2251-9637
2251-9645
10.22088/IJMCM.BUMS
en
jalali
1393
6
1
gregorian
2014
9
1
3
4
online
1
fulltext
en
Cytotoxic Activity of Bioactive Compound 1, 2- Benzene Dicarboxylic Acid, Mono 2- Ethylhexyl Ester Extracted from a Marine Derived Streptomyces sp. VITSJK8
Other
Other
Original Article
Original Article
Marine Streptomyces are prolific producers of majority of bioactive secondary metabolites which are used in pharmaceutical industry as effective drugs against life threatening diseases. The cytotoxic activity of the pure compound 1, 2- benzene dicarboxylic acid, mono 2- ethylhexyl ester (DMEHE) from marine derived actinomycete Streptomyces sp. VITSJK8 was investigated against mouse embryonic fibroblast (NIH 3T3) and human keratinocyte (HaCaT) normal cell lines, human hepatocellular liver carcinoma (HepG 2) and human breast adenocarcinoma (MCF-7) cell lines by using MTT assay. The compound DMEHE exhibited IC 50 values of 42, 100, 250 and 500 µg/ ml against HepG2, MCF-7, HaCaT and NIH 3T3 cell lines, respectively. The effect of DMEHE on the growth of cancer cell lines was expressed as the % of viability. Cell viability was recorded as 67.7%, 78.14%, 82.23% and 96. 11% in HepG2, MCF-7, HaCaT and NIH 3T3 cells, respectively. The results of the study conclude that the bioactive compound isolated from the potential isolate Streptomyces sp. VITSJK8 exhibited cytotoxic activity against HepG2 and MCF- 7 cancer cell lines and low toxicity against normal HaCaT and NIH 3T3 cell lines.
Streptomyces sp. VITSJK8, 1, 2- benzene dicarboxylic acid, mono 2- ethylhexyl ester, cytotoxicity, MTT assay
246
254
http://ijmcmed.org/browse.php?a_code=A-10-245-2&slc_lang=en&sid=1
Kannabiran
Krishnan
kkb@vit.ac.in
10031947532846004109
10031947532846004109
Yes
School of Biosciences and Technology, VIT University, Tamilnadu, India.
Abirami
Mani
abi.biotechnology@gmail.com
10031947532846004110
10031947532846004110
No
School of Biosciences and Technology, VIT University, Tamilnadu, India.
Subashini
Jasmine
subajes@gmail.com
10031947532846004111
10031947532846004111
No
School of Biosciences and Technology, VIT University, Tamilnadu, India.