en Hematology Hematology Original Article Original Article <span style="font-size:12px;"><span style="font-family:Tahoma;"><span style="unicode-bidi:embed"><span style="line-height:107%">Acute myeloid leukemia (AML) is an invasive form of hematologic malignancies which results in the overproduction of myeloid cells in the bone marrow. Aberrant expression of piwi-interacting RNAs (piRNAs) which belong to small non-coding RNAs, play important roles in different cancer cells&#39; progress. hsa- piR- 32877 is up-regulated in AML. Down regulation of hsa-piR-32877 by antisense LNA GapmeRs could be potential for suppression of myeloid cell proliferation and induce myeloid cell apoptosis. We have blocked the expression of hsa-piR-32877 by antisense LNA GapmeRs in human bone marrow blast cells, and the M-07e cell line. Samples were transfected with antisense LNA GapmeRs at 24, 48, and 72 hours. The Quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) was performed to investigate the expression of hsa-piR-32877, CASP3, and CASP9. Both CASP3 and CASP9 play important roles in apoptosis. Cell proliferation was studied via CFSE (carboxyfluorescein diacetate succinimidyl ester) assay. Results showed that hsa-piR-32877 was down-regulated by antisense LNA GapmeRs in the patient and cell line samples. Also, after transfection, cell proliferation and apoptosis decreased and increased, respectively. Our data suggested that hsa-piR-32877 suppression may act as a novel therapeutic method for the inhibition of human leukemic cells proliferation in AML<span dir="RTL" lang="FA">.</span></span></span></span></span> Acute myeloid leukemia, Antisense LNA GapmeRs, hsa-piR-32877, CASP3, CASP9 18 29 http://ijmcmed.org/browse.php?a_code=A-10-1083-3&slc_lang=en&sid=1 Sepideh Naseri sepideh1374nasseri@gmail.com 100319475328460032670 100319475328460032670 No Department of Genetics and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran. Mohammadreza Sharifi mo_sharifi@med.mui.ac.ir 100319475328460032671 100319475328460032671 Yes Department of Genetics and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran. Valiollah Mehrzad v_mehrzad@med.mui.ac.ir 100319475328460032672 100319475328460032672 No Department of Internal Medicine, Division of Hematology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.