en Cancer Cancer Original Article Original Article <span style="font-size:12px;"><span style="line-height:200%"><span sans-serif="" style="font-family:Calibri,"><span style="line-height:200%"><span new="" roman="" style="font-family:" times="">Among HPV-mediated cervical cancers, cellular factor BRN3A has gained considerable attention due to its role in promoting an anti-apoptotic cellular environment and in facilitating epitheliotropic transformations of the host. The majority of previous studies looked at BRN3A&#39;s molecular characteristics; however, the possibility of genetic variations in BRN3A&#39;s auto-regulatory region in relation to cervical cancer risk has been underestimated until now. In a retrospective study in the Eastern UP population, India, we detected genetic variations in the cis-regulatory proximal enhancer region located around 5.6 kb upstream of transcription start site of <i>BRN3A</i>. Our analysis of PCR and DNA sequencing confirmed this novel SNP (<i>BRN3A </i>g.60163379A>G) within the auto-regulatory region of <i>BRN3A</i>. As compared to control subjects, cancer cases exhibited a 1.32-fold higher allele frequency (&chi;2 = 6.315, <i>p </i>= 0.012). In homozygous (GG) but not in heterozygous conditions, odds ratio (OR) analysis suggests a significant association of cancer risk with the SNP (OR = 2.60, p &le; 0.004). We further confirmed using the functional analysis that this SNP increased the luciferase gene activity in HPV-positive cervical cancer SiHa cells that were exposed to progesterone. As a result of the association of polymorphisms in a non-coding region of an oncogene with increased cancer risks, we are suggesting that this genetic variation in non-coding region can be used in prediction, diagnosis, or predicting the progression of the disease.</span></span></span></span></span> Cervical cancer, HPV, BRN3A, BRN3A proximal enhancer region, India, g.60163379A>G 88 103 http://ijmcmed.org/browse.php?a_code=A-10-40-5&slc_lang=en&sid=1 Anand Prakash anand.days@gmail.com 100319475328460026136 100319475328460026136 No Department of Zoology, Banaras Hindu University, Varanasi, India. Biswa Pratim Das Purkayastha bhishmapratim@gmail.com 100319475328460026137 100319475328460026137 No Department of Zoology, Banaras Hindu University, Varanasi, India. Shikha Srivastava shikha_srivastava2003@yahoo.co.in 100319475328460026138 100319475328460026138 No Department of Zoology, Banaras Hindu University, Varanasi, India. Sunanda Chaturvedi sunanda.chaturvedi@gov.in 100319475328460026139 100319475328460026139 No Homi Bhabha Cancer Hospital & Research Centre, Varanasi, India. Akhtar Ali akhtar@bhu.ac.in 100319475328460026140 100319475328460026140 No Centre for Genetic Disorders, Banaras Hindu University, Varanasi, India. Dau Dayal Aggarwal ddgenetics@gmail.com 100319475328460026141 100319475328460026141 No Department of Zoology, Banaras Hindu University, Varanasi, India. Jagat Kumar Roy jkroy@bhu.ac.in 100319475328460026142 100319475328460026142 Yes Department of Zoology, Banaras Hindu University, Varanasi, India.