en Hematology Hematology Original Article Original Article <span style="font-size:12px;"><span style="line-height:150%"><span sans-serif="" style="font-family:Calibri,"><span class="fontstyle01" serif="" style="font-family:Rpxr,"><span style="color:black"><span style="font-weight:normal"><span style="font-style:normal"><span style="line-height:150%">Angiogenesis is an essential process in the growth, development and transition of tumors from dormancy to proliferating state. Resveratrol (RSV), as a natural polyphenolic compound, is claimed to be effective in angiogenesis regulation. The aim of</span></span></span></span></span><span style="line-height:150%"><span new="" roman="" style="font-family:" times=""> this study was to evaluate the impact of RSV on angiogenesis process in human umbilical vein endothelial cells (HUVECs) alone and co-cultured with Jurkat cells.<b> </b>The effects of RSV on HUVECs and Jurkat cells viability and apoptosis were measured by MTT and annexin-V/PI methods. HUVECs were co-cultured with pre-treated Jurkat cells and incubated for 24-72 h. Angiogenesis was also investigated by analyzing the expression of</span></span> <span style="line-height:150%"><span new="" roman="" style="font-family:" times="">VEGF, VEGFR-2, and IL-8 employing qPCR and ELISA.<b> </b>Treatment with Jurkat cells had no significant effect on apoptosis of HUVECs at low concentrations of RSV (20-40 &micro;M), but higher concentrations (80-160 &micro;M) increased apoptosis. Although RSV significantly reduced <i>VEGFR2</i> and <i>IL-8</i> gene expression as well as IL-8 protein concentration in HUVECs, this effect was reversed in the Jurkat-treated HUVECs in the co-culture model. The expression of <i>VEGF</i> in Jurkat cells significantly increased following treatment with RSV.<b> </b>Therefore, RSV had a direct antiangiogenic effect on HUVECs. Unexpectedly its indirect effect was not significant on Jurkat-treated HUVECs in co-culture conditions. The results of our experiments highlight that although </span></span><span lang="EN"><span style="line-height:150%"><span new="" roman="" style="font-family:" times="">RSV may be effective in antiangiogenesis therapy, </span></span></span><span style="line-height:150%"><span new="" roman="" style="font-family:" times="">in some conditions it may favor angiogenesis. Therefore, appropriate concentrations should be achieved to minimize the possible unpredicted effects of RSV.</span></span></span></span></span> Angiogenesis, resveratrol, co-culture, VEGFR2, VEGF, IL-8 16 30 http://ijmcmed.org/browse.php?a_code=A-10-4672-1&slc_lang=en&sid=1 Pegah Kiyamehr kiyamehr72pk@yahoo.com 100319475328460026109 100319475328460026109 No Department of Hematology and Blood Banking, School of Allied Medical Sciences, Iran University of Medical Sciences, Tehran, Iran. Minoo Shahidi shahidi.minoo@gmail.com 100319475328460026110 100319475328460026110 Yes Department of Hematology and Blood Banking, School of Allied Medical Sciences, Iran University of Medical Sciences, Tehran, Iran. Amir Samii amirsamie70.as@gmail.com 100319475328460026111 100319475328460026111 No Department of Hematology and Blood Banking, School of Allied Medical Sciences, Iran University of Medical Sciences, Tehran, Iran. Farhad Zaker farhadz20@yahoo.co.uk 100319475328460026112 100319475328460026112 No Department of Hematology and Blood Banking, School of Allied Medical Sciences, Iran University of Medical Sciences, Tehran, Iran.