en Biomarkers (diagnosis & treatment) Biomarkers (diagnosis & treatment) Original Article Original Article <div style="text-align: justify;">In this study, a sensitive and accurate aptasensor was designed for early detection of myocardial infarction through the determination of troponin T (TnT). The successful immobilization of a specific aptamer sequence on the surface of gold that had a high affinity toward TnT was accomplished. TnT was electrochemically quantified. The results indicated that the aptasensor detected TnT in a range of 0.05-5 ng mL and with a detection limit of 0.01 ng/mL. The performance of the aptasensor was investigated by analyzing 99 human serum samples. Both diagnostic specificity and sensitivity of the aptasensor were found to be 95%. The use of the designed aptamer-based biosensor could be an essential achievement in health policy, preventing deaths caused by myocardial infarction, and reducing patients with heart failure. The extensive use of this aptamer-based biosensor can also reduce costs, enhance speed, and improve accuracy in the diagnosis of TnT as an important myocardial infarction biomarker.</div> Myocardial infarction, aptasensing, biomarker, bioelectrochemical detection 271 282 http://ijmcmed.org/browse.php?a_code=A-10-2256-1&slc_lang=en&sid=1 Masoud Negahdary masoudnegahdary@yahoo.com 100319475328460016647 100319475328460016647 No Nanomedicine and Nanobiology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran; Yazd Cardiovascular Research Center, Shahid Sadoughi University of Medical Sciences, Yazd, Iran. Mostafa Behjati-Ardakani dr_behjati@yahoo.com 100319475328460016648 100319475328460016648 No Yazd Cardiovascular Research Center, Shahid Sadoughi University of Medical Sciences, Yazd, Iran. Hossein Heli hheli7@yahoo.com 100319475328460016649 100319475328460016649 No Nanomedicine and Nanobiology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran. Naghmeh Sattarahmady nsattar@sums.ac.ir 100319475328460016650 100319475328460016650 Yes Nanomedicine and Nanobiology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran; Department of Medical Physics, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.