International Journal of Molecular and Cellular Medicine
مجله بین المللی سلولی و مولکولی
Int J Mol Cell Med
Medical Sciences
http://ijmcmed.org
1
admin
2251-9637
2251-9645
10.22088/IJMCM.BUMS
en
jalali
1398
4
1
gregorian
2019
7
1
8
2
online
1
fulltext
en
Migration Gene Expression of Human Umbilical Cord Mesenchymal Stem Cells: A Comparison between Monophosphoryl Lipid A and Supernatant of Lactobacillus acidophilus
Stem Cell
Stem Cell
Original Article
Original Article
<div style="text-align: justify;">The capacity of human umbilical cord mesenchymal stem cells (hUMSCs) for migration and homing is very important in regenerative medicine. A detoxified derivative of lipopolysaccharides (LPS) that lacks many of the endotoxic properties of LPS is monophosphoryl lipid A (MPLA). Effects of MPLA on the induction of MSCs migration, have not yet been studied. Also, studies have shown that supernatant of <em>Lactobacillus acidophilus</em> (SLA) culture medium, can stimulate the proliferation of macrophages and lymphocytes <em>in vitro</em> by affecting the properties of the chemotaxis and angiogenesis. Our present study aimed to understanding of the migration of hUMSCs during treatment with MPLA and LS, separately. HUMSCs were isolated from human umbilical cord and were characterized by investigating surface markers (CD105, CD90, anti-CD29, CD45, and CD34) and their differentiation into adipogenic and osteogenic lineages. HUMSCs were treated with MPLA (10<sup>-3</sup> µg/ml) and SLA (3 µl/ml). The morphological changes were not observed in both treated MSCs. <em>Expression </em><em>levels</em> of migration <em>markers were </em>determined by quantitative reverse transcription PCR analysis on 2, 4, 6 days after treatment. Results showed that <em>VEGF</em> and <em>MMP-2</em> but not <em>CXCR-4</em> was increased in the presence of both treatments. Also, SLA led to a decrease and increase of the expression of <em>VLA-4</em> and <em>VCAM-1</em>, respectively, while MPLA increased both <em>VLA-4</em> and <em>VCAM-1</em> expression.Therefore, it can be suggested that MPLA has more prominent results than SLA, but both treatments can probably be considered as an inducing factor in migration.</div>
Human umbilical cord mesenchymal stem cells, monophosphoryl lipid A, Lactobacillus acidophilus, VCAM-1
154
161
http://ijmcmed.org/browse.php?a_code=A-10-1640-2&slc_lang=en&sid=1
Ali
Shojaeian
ali.shojaeian65@gmail.com
100319475328460015601
100319475328460015601
No
Department of Molecular Medicine, School of Advanced Technologies, Shahrekord University of Medical Sciences, Shahrekord, Iran.
Ameneh
Mehri-Ghahfarrokhi
ameneh.mehri.96@gmail.com
100319475328460015602
100319475328460015602
No
Department of Molecular Medicine, School of Advanced Technologies, Shahrekord University of Medical Sciences, Shahrekord, Iran.
Mehdi
Banitalebi-Dehkordi
mehdibanitalebi@gmail.com
100319475328460015603
100319475328460015603
Yes
Cellular and Molecular Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, Iran