en Nanomedicine Nanomedicine Original Article Original Article <div style="text-align: justify;"><a name="OLE_LINK1">Nanofiber scaffolds </a>and bio-ceramic nanoparticles have been widely used in bone tissue engineering. The use of human induced <a name="OLE_LINK2">pluripotent</a> stem cells (<a name="OLE_LINK7">hiPSCs</a>) on this <a name="OLE_LINK3">scaffold </a>can be considered as a new approach in the differentiation of bone tissue. In the present study, a polyaniline-gelatin-polycaprolactone <a name="OLE_LINK69">(PANi-GEL-PCL)</a> composite <a name="OLE_LINK5">nanoscaffold </a>was made by electrospinning and modified superficially by plasma method. The synthesized <a name="OLE_LINK6">nanoscaffold </a>was then coated with <a name="OLE_LINK4"></a><a name="OLE_LINK29">willemite&#39;s </a>bio-ceramic nanoparticles (Zn₂SiO₄). The nanoscaffold&rsquo;s properties were studied by scanning electron microscopy (SEM). Also, nanoparticles characterization was carried out with SEM and dynamic light scattering. The growth and proliferation rate of cells on the synthesized nanoscaffold was examined by MTT assay. Subsequently, hiPSCs were cultured on murine fibroblast cells, incubated in embryoid bodies for 3 days, and placed on the nanoscaffolds. The differentiation potential of hiPSCs was investigated by examination of common bone markers (e.g. alkaline phosphatase, calcium salt precipitation, and <a name="OLE_LINK9">alizarin red test</a>) using bone differentiation factors for 14 days. SEM showed the proper structure of electrospinned nanoscaffolds and coating of nanoparticles on the <a name="OLE_LINK10">nanoscaffold </a>surface. The results of MTT assay confirmed the growth and proliferation of cells and the biocompatibility of nanofibers. The results of bone indices also showed that differentiation on the composite <a name="OLE_LINK11">nanoscaffold </a>coated with <a name="OLE_LINK12">willemite&#39;s bio-ceramic </a>nanoparticles was dramatically increased in comparison with other groups. Overall, this study demonstrated that PANi-GEL-PCL composite nanoscaffold with willemite&#39;s bio-ceramic nanoparticles is a suitable substrate for <em>in vitro </em>growth, proliferation, and differentiation of hiPSCs cells into osteoblasts.</div> Osteoblast differentiation, human induced pluripotent stem cells, poly-aniline-gelatin-polycaprolactone, Zn2SiO4 bio-ceramic nanoparticle 24 38 http://ijmcmed.org/browse.php?a_code=A-10-1794-1&slc_lang=en&sid=1 Raheleh Halabian r.halabian@yahoo.com 100319475328460015989 100319475328460015989 No Applied Microbiology Research Center, Systems Biology and Poisonings Institute, Baqiyatallah University of Medical Sciences, Tehran, Iran. Ali Salimi salimi.ali@bmsu.ac.ir 100319475328460015990 100319475328460015990 No Nano biotechnology Research Center, Ba qiyatallah University of Medical Sciences, Tehran, Iran. Kaykhosro Moridi moridi@gmail.com 100319475328460015991 100319475328460015991 No Department of Medical Nanotechnology, Faculty of Advanced Sciences & Technology, Pharmaceutical Sciences Branch, Islamic Azad U niversity (IAUPS), Tehran, Iran. Masoumeh Dodel dde@gmail.com 100319475328460015992 100319475328460015992 No Textile Engineering Department, Amirkabir University of Technology, Tehran, Iran. Mohsen Korani korani@yahoo.com 100319475328460015993 100319475328460015993 No Chemical Injuries Research Center, Systems Biology and Poisonings Institute, Baqiyatallah University of Medical Sciences, Tehran, Iran. Marzieh Ghollasi ghollasi@khu.ac.ir 100319475328460015994 100319475328460015994 Yes Department of Cell and Molecular Biology, Faculty of Biological Science, Kharazmi University, Tehran, Iran.