TY - JOUR T1 - Determination of ctxAB expression in Vibrio cholerae Classical and El Tor strains using Real-Time PCR TT - JF - ijmcmed JO - ijmcmed VL - 2 IS - 1 UR - http://ijmcmed.org/article-1-73-en.html Y1 - 2013 SP - 9 EP - 13 KW - Vibrio cholerae KW - RT-qPCR KW - ctxAB expression N2 - Cholera is an infection of the small intestines caused by the bacterium V. cholerae. It is a major cause of health threat and also a major cause of death worldwide and especially in developing countries. The major virulence factor produced by V. cholerae during infection is the cholera toxin. Total mRNA extraction and reverse transcription was performed for making ctxAB cDNA. Relative Real-Time PCR analysis showed unequal enterotoxin production in V. cholerae strains. The results showed that, classical strain produces cholera toxin more than El Tor strain. M3 ER -