@ARTICLE{Omrani, author = {Fazeli, Zahra and Rajabibazl, Masoumeh and Salami, Siamak and Vazifeh Shiran, Nader and Ghaderian, Sayyed Mohammad Hossein and Omrani, Mir Davood and }, title = {Expression Pattern of Neuronal Markers in PB-MSCs Treated by Growth Factors Noggin, bFGF and EGF}, volume = {4}, number = {4}, abstract ={Mesenchymal stem cells (MSCs) have the ability to differentiate into neuronal like cells under appropriate culture condition. In this study, we investigated whether MSCs derived from human peripheral blood (PB-MSCs) can differentiate into neuronal like cells by synergic effect of the growth factors EGF, bFGF and Noggin. For this purpose, the expression of five neuronal markers (Nestin, &beta III tubulin, NFM, MAP2 and NSE) were evaluated in treated PB-MSCs by SYBR Green Real time PCR. The expression analysis showed a higher expression of &beta- tubulin and NFM in treated BP-MSCs compared with untreated PB-MSCs as a control group. The expression of Nestin was also diminished in PB-MSCs treated with Noggin. This study suggested that the treatment of PB- MSCs with Noggin alongside with bFGF and EGF might differentiate these cells into neuronal lineage cells. The obtained results could be further developed for useful applications in regenerative medicine. }, URL = {http://ijmcmed.org/article-1-384-en.html}, eprint = {http://ijmcmed.org/article-1-384-en.pdf}, journal = {International Journal of Molecular and Cellular Medicine}, doi = {}, year = {2015} }