TY - JOUR T1 - miR-33-5p Regulates CREB to Induce Morphine State-dependent Memory in Rats: Interaction with ยต Opioid Receptor TT - JF - ijmcmed JO - ijmcmed VL - 11 IS - 2 UR - http://ijmcmed.org/article-1-1930-en.html Y1 - 2022 SP - 150 EP - 167 KW - miR-33-5p KW - CREB KW - state-dependent memory KW - opioids KW - rats N2 - The aim of the present study was to examine the hypothesis that miR-33-5p attenuates morphine state-dependent (StD) memory via the µ opioid receptor by regulating cyclic AMP response element-binding protein (CREB). The effects of post-training morphine and morphine StD memory and their interaction with pre-test naloxone were evaluated using a single-trial inhibitory avoidance paradigm. Then, the hippocampal miR-33-5p gene and pCREB/CREB protein expression profiles were evaluated using quantitative real-time PCR and western blotting, respectively. We found that while post-training morphine and morphine StD memory respectively up- and down-regulate the miR-33-5p expression profile in the hippocampus, the reverse results are true for the expression of pCREB/CREB. Pre-test naloxone antagonized the response. Overall, our findings suggest that the expression levels of miR-33-5p in the hippocampus set the basis for morphine StD memory with low miR-33-5p enabling state dependency. The mechanism is mediated via miR33-5p and CREB signaling with the interaction of the µ opioid receptor. This finding may be used as a potential strategy for ameliorating morphine-induced memory-related disorders. M3 10.22088/IJMCM.BUMS.11.2.150 ER -