TY - JOUR JF - ijmcmed JO - Int J Mol Cell Med VL - 3 IS - 4 PY - 2014 Y1 - 2014/9/01 TI - Achillea Millefolium L. Hydro- Alcoholic Extract Protects Pancreatic Cells by Down Regulating IL- 1β and iNOS Gene Expression in Diabetic Rats TT - N2 - Interleukin-1β (IL-1β) has a role in β- cell destruction in autoimmune diabetes by stimulating the expression of inducible nitric oxide synthase (iNOS) that generates the free radical nitric oxide. We aimed to investigate the effect of Achillea millefolium L, as a traditional hypoglycemic agent, on IL-1β and iNOS gene expression of pancreatic tissue in the STZ- induced diabetic rats. Forty adult male Wistar rats were randomly divided into four groups: 1. diabetic control 2. diabetic rats treated with Achillea millefolium L. extract 3. normal rats received only extract and 4. negative control (n= 10 each). Diabetes was induced by single i.p. injection of 45 mg/ kg streptozotocin (STZ). Rats in groups 2 and 3 were treated with i.p. injection of Achillea millefolium L. extract (100 mg/ kg/ day) for 14 days. Body weight, serum glucose and insulin levels were assayed at baseline and on days 3, 7, 10 and 14 of the experiment. Finally, the quantity of pancreatic IL-1β and iNOS mRNA was determined by real- time PCR. The mRNA expression level of IL-1β and iNOS genes, was significantly (p SP - 255 EP - 262 AU - Zolghadri, Yalda AU - Fazeli, Mehdi AU - Kooshki, Marzieh AU - Shomali, Tahoora AU - Karimaghayee, Negar AU - Dehghani, Maryam AD - Division of Pharmacology and Toxicology, Department of Basic Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, Iran. KW - Achillea millefolium L. KW - IL-1 KW - iNOS KW - diabetes KW - gene expression UR - http://ijmcmed.org/article-1-221-en.html ER -