TY - JOUR JF - ijmcmed JO - Int J Mol Cell Med VL - 8 IS - 4 PY - 2019 Y1 - 2019/11/01 TI - Proliferation, Characterization and Differentiation Potency of Adipose Tissue-Derived Mesenchymal Stem Cells (AT-MSCs) Cultured in Fresh Frozen and non-Fresh Frozen Plasma TT - N2 - Mesenchymal stem cells (MSCs) have unique properties, including high proliferation rates, self-renewal, and multilineage differentiation ability. Their characteristics are affected by increasing age and microenvironment. This research aimed to determine the proliferation, characteristics and differentiation capacity of adipose tissue-derived (AT)-MSCs at many passages with different media. The cell proliferation capacity was assayed using trypan blue. MSCs characterization (CD90, CD44, CD105, CD73, CD11b, CD19, CD34, CD45, and HLA-DR) was performed by flow cytometry, and cell differentiation was determined by specific stainings. Population doubling time (PDT) of AT-MSCs treated with fresh frozen plasma (FFP) and non-FFP increased in the late passage (P) (P15 FFP was 22.67 ± 7.01 days and non-FFP was 19.65 ± 2.27 days). Cumulative cell number was significantly different between FFP and non-FFP at P5, 10, 15. AT-MSCs at P4-15 were positive for CD90, CD44, CD105, and CD73, and negative for CD11b, CD19, CD34, CD45, and HLA-DR surface markers. AT-MSCs at P5, 10, 15 had potential toward adipogenic, chondrogenic, and osteogenic differentiation. Therefore, PDT was affected by increased age but no difference was observed in morphology, surface markers and differentiation capacity among passages. Cumulative cell number in FFP was higher in comparison with non-FFP in P5, 10, 15. Our data suggest that FFP may replace FBS for culturing MSCs. SP - 283 EP - 293 AU - Widowati, Wahyu AU - Noverina, Rachmawati AU - Ayuningtyas, Wireni AU - Kurniawan, Dedy AU - Sari Widya Kusuma, Hanna AU - Arumwardana, Seila AU - Surya Artie, Dwi AU - Adhani Sholihah, Ika AU - Siwianti Handayani, Rr. Anisa AU - Ratih Laksmitawati, Dian AU - Rinendyaputri, Ratih AU - Rilianawati, Rilianawati AU - Faried, Ahmad AD - Medical Research Center, Faculty of Medicine, Maranatha Christian University, Bandung, West Java, Indonesia KW - AT-MSCs KW - multilineage differentiation KW - PDT KW - proliferation KW - surface marker UR - http://ijmcmed.org/article-1-1215-en.html DO - 10.22088/IJMCM.BUMS.8.4.283 ER -