:: Volume 7, Issue 4 (Int J Mol Cell Med [In press] 2018) ::
Int J Mol Cell Med 2018, 7(4): 0-0 Back to browse issues page
Evaluating the Expression of NOX2 and NOX4 Signaling Pathways in Rats’ Lung Tissues Following Local Chest Irradiation; Modulatory Effect of Melatonin
Masoud Najafi1, Alireza Shirazi 2, Elahe Motevaseli3, Ghazale Geraily1, Peyman Amini4, Dheyauldeen Shabeeb5, Ahmed Eleojo Musa6
1- Department of Medical Physics and Biomedical Engineering, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
2- Department of Medical Physics and Biomedical Engineering, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran. , shirazia@sina.tums.ac.ir
3- Department of Molecular Medicine, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran.
4- Department of Radiology, Faculty of Paramedical, Tehran University of Medical Sciences, Tehran, Iran.
5- Department of Medical Physics and Biomedical Engineering, Faculty of Medicine, Tehran University of Medical Sciences (International Campus), Tehran, Iran.
6- Research center for molecular and cellular imaging, Tehran University of Medical Sciences (International Campus), Tehran, Iran.
Abstract:   (1213 Views)
Lung injury is one of the major concerns for chest cancer patients that undergo radiotherapy as well as persons exposed to an accidental radiological event. Reduction/oxidation (redox) system plays a key role in lung injury via chronic upregulation of pro-oxidant enzymes. NOX2 and NOX4 are two important reactive oxygen species generating enzymes that are involved in radiation toxicity in some organs such as the bone marrow. In this study, we aimed to evaluate the expression of NOX2 and NOX4 signaling in rat’s lung tissues. Upregulation of these genes may be involved in radiation-induced lung injury. Moreover, we evaluated the role of pre-treatment with melatonin on the expression of these genes. Twenty male rats were divided into 4 groups as control; melatonin treated; irradiation; and irradiation with melatonin pre-treatment. Rats were exposed to 15 Gy 60Co gamma rays and sacrificed after 10 weeks for evaluation of NF-κB, TGFbR1, SMAD2, NOX2, and NOX4 genes expression by real-time PCR. Results showed the upregulation of all five genes. The expression of NOX2 was more obvious compared to other genes. Administration of melatonin before irradiation could attenuate the expression of all mentioned genes. Results indicate that upregulation of NADPH oxidase genes such as NOX2 and NOX4 may be involved in the late effects of lung exposure to ionizing radiation. Melatonin via downregulation of these pro-oxidant genes is able to attenuate radiation toxicity in the lung.
Keywords: Radiation, Lung, NOX2, NOX4, TGFR1, SMAD2
Full-Text [PDF 103 kb]   (82 Downloads)    
Type of Study: Original Article | Subject: Biomarkers (diagnosis & treatment)
Received: 2018/10/10 | Accepted: 2018/11/12 | Published: 2018/11/12


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