:: Volume 7, Issue 3 (Int J Mol Cell Med 2018) ::
Int J Mol Cell Med 2018, 7(3): 176-184 Back to browse issues page
Effects of Resveratrol on FOXO1 and FOXO3a Genes Expression in Adipose Tissue, Serum Insulin, Insulin Resistance and Serum SOD Activity in Type 2 Diabetic Rats
Soheila Asadi1, Zohreh Rahimi1, Massoud Sadijam2, Nooshin Shabab2, Mohammad Taghi Goodarzi 3
1- Department of Clinical Biochemistry, Faculty of Medicine, Kermanshah University of Medical Sciences, Kermanshah-Iran.
2- Research Center for Molecular Medicine, Hamadan University of Medical Sciences, Hamadan, Iran.
3- Research Center for Molecular Medicine, Hamadan University of Medical Sciences, Hamadan, Iran. , mtgoodarzi@yahoo.com
Abstract:   (1143 Views)

Induced oxidative stress in diabetes mellitus (DM) plays a critical role in insulin resistance. Fork head-related transcription factor (FOXO) proteins are important transcriptional factors involved in oxidative stress and insulin resistance. Resveratrol (RSV) is a polyphenol with hypoglycemic and antioxidant properties. The aims of the present study were to examine the effects of RSV on FOXO gene expression, serum superoxide dismutase (SOD) activity, insulin level, and insulin resistance in type 2 diabetic (T2DM) rats. Thirty male Wistar rats were used in this study. DM was induced in rats (n=24) using streptozotocin (STZ) and nicotinamide; then, they were divided into 4 groups of 6 rats each. Six untreated normal rats were used as normal control group; diabetic rats in groups 2 to 5 were treated with 0, 1, 5 and 10 mg /kg body weight of RSV, respectively for 30 days. At the end of the experimental period, the rats were sacrificed, their sera were separated, and adipose tissues were obtained and stored at −80 °C. Serum glucose and SOD activity levels were determined biochemically, and serum insulin level was determined by ELISA method. Gere expression in FOXO1 and FOXO3a in adipose tissue was evaluated using real‐time PCR. Results indicated that RSV significantly reduced blood glucose level, increased insulin level and improved insulin sensitivity. RSV resulted in an increased serum SOD activity and caused decreased FOXO1 and FOXO3a expression in adipose tissue of rats with T2DM. Therefore, by attenuation of FOXO expression in adipose tissue of T2DM rats, RSV showed a hypoglycemic potential and antioxidant properties, and consequently ameliorated insulin resistance.

Keywords: Oxidative stress, diabetes mellitus, FOXO, insulin resistance, resveratrol, superoxide dismutase
Full-Text [PDF 461 kb]   (382 Downloads)    
Type of Study: Original Article | Subject: Clinical Biochemistry
Received: 2018/09/2 | Accepted: 2018/11/12 | Published: 2019/01/22

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