:: Volume 7, Issue 3 (Int J Mol Cell Med 2018) ::
Int J Mol Cell Med 2018, 7(3): 146-157 Back to browse issues page
Simulated Microgravity Condition Alters the Gene Expression of some ECM and Adhesive Molecules in Adipose-Derived Stem Cells
Farid Ebnerasuly1, Zahra Hajebrahimi 2, Seyed Mehdi Tabaie3, Mojtaba Darbouy1
1- Department of Biology, Fars Science and Research Branch, Islamic Azad University, Marvdasht, Iran.
2- Aerospace Research Institute, Ministry of Science Research and Technology, Tehran, Iran. , hajebrahimi@ari.ac.ir
3- Department of Photo Healing and Regeneration, Medical Laser Research Center, Yara Institute, ACECR, Tehran, Iran.
Abstract:   (1064 Views)
Adipose-derived stem cells (ADSCs) are widely used for tissue engineering and regenerative medicine. The beneficial effects of ADSCs on wound healing have already been reported. Remodeling of extracellular matrix (ECM) is the most important physiological event during the wound healing. ECM is sensitive to mechanical stresses and the expression of its components can be therefore influenced. The aim of this study was to investigate the effect of simulated microgravity on the gene expression of some ECM and adhesion molecules in human ADSCs. After isolation and characterization of ADSCs, cells were exposed to simulated microgravity for 1, 3 and 7 days.  Real-time PCR, fluorescence immunocytochemistry, and MTT assay were performed to evaluate the alterations of integrin subunit beta 1 (ITGB1), collagen type 3 (ColIII), matrix metalloproteinase-1 (MMP1), CD44, fibrillin (FBN1), vimentin (VIM) genes, and ColIII protein levels as well as cells viability. Microgravity simulation increased the expression of ITGB1, ColIII, MMP1, and CD44 and declined the expression of FBN1 and VIM genes. ColIII protein levels were also increased.  There were no significant changes in the viability of cells cultured in microgravity. Since the high expression of ECM components is known as one of the fibroblast markers, our data suggest that pretreatment of ADSCs by simulated microgravity may increase their differentiation capacity towards fibroblastic cells. Microgravity had not adversely affected the viability of ADSCs, and it is likely to be used alone or in combination with biochemical inducers for cell manipulation.
Keywords: Adipose derived stem cells, simulated microgravity, extracellular matrix, adhesion
Full-Text [PDF 661 kb]   (253 Downloads)    
Type of Study: Original Article | Subject: Stem Cell
Received: 2018/05/30 | Accepted: 2018/09/27 | Published: 2018/10/8

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Volume 7, Issue 3 (Int J Mol Cell Med 2018) Back to browse issues page