:: Volume 7, Issue 2 (Int J Mol Cell Med 2018) ::
Int J Mol Cell Med 2018, 7(2): 133-145 Back to browse issues page
Screening for Non-polyenic Antifungal Produced by Actinobacteria from Moroccan Habitats: Assessment of Antimycin A19 Production by Streptomyces albidoflavus AS25
Ahmed Nafis 1, Najoua Elhidar2, Brahim Oubaha2, Salah eddine Samri3, Timo Niedermeyer4, Yedir Ouhdouch2, Lahcen Hassani2, Mustapha Barakate Barakate2
1- Laboratory of Biology and Biotechnology of Microorganisms, Faculty of Sciences Semlalia, Cadi Ayyad University, Marrakech, Morocco. , ahmed.nafis@edu.uca.ac.ma
2- Laboratory of Biology and Biotechnology of Microorganisms, Faculty of Sciences Semlalia, Cadi Ayyad University, Marrakech, Morocco.
3- Department of Biology, Nador Multidisciplinary Faculty, Mohamed First University, Nador, Morocco.
4- Interfaculty Institute of Microbiology and Infection Medicine (IMIT), Eberhard Karls University Tübingen, Tübingen, Germany.
Abstract:   (4773 Views)
Fungal diseases are currently a serious public health problem, due to the limited number of really effective principles, and the emergence of resistant strains to the polyenic antifungals. The aim of this study was to screen, for non-polyenic antifungals production by Actinobacteria, and to validate the screening program by characterizing the produced compounds. Actinobacteria isolates were tested against four clinic human pathogenic fungi isolated from Hospital Mohammed V Rabat, Morocco. The production of non-polyenic antifungal metabolites by active isolates was investigated based on the yeast cell specificity as challenging targets, antibacterial activity, activity against resistant Candida tropicalis R2 and Pythium irregular (resistant to polyenes), inhibition of antifungal activity by the addition of exogenous ergosterol, and the UV-visible light spectrophotometric analysis of the active crude extracts. The antifungal compound produced was purified using various chromatographic techniques and the selected producing strain was identified using the polyphasic approach. Among 480 Actinobacteria isolates, 55 showed antifungal activity against all tested clinically derived fungi. After performing the screening program, 4 Actinobacteria that had all the desired criteria were selected. Using the polyphasic approach, the taxonomic position of the selected  Streptomyces AS25, isolated from rhizospheric soil of Alyssum spinosum, showed that it belongs to Streptomyces genus with 100% partial 16S similarity with Streptomyces albidoflavus NBRC13010. On the basis of HPLC and mass spectrometry, the purified compound produced by Streptomyces AS25 was identified as a non-polyenic lactone, antimycin A19, which has been found for the first time to be produced by Streptomyces albidoflavus strain. Following the obtained results, it is important to note that our screening criteria for non-polyenic antifungals have been validated and the rhizospheric soil represent an interesting source to isolate Actinobacteria.
Keywords: Validation, program screening, non-polyenic antifungal, Actinomycetes, characterization, Antimycin A19
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Type of Study: Original Article | Subject: Medical Pharmacology
Received: 2018/02/23 | Accepted: 2018/06/3 | Published: 2018/06/3

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Volume 7, Issue 2 (Int J Mol Cell Med 2018) Back to browse issues page