:: Volume 7, Issue 1 (Int J Mol Cell Med 2018) ::
Int J Mol Cell Med 2018, 7(1): 8-16 Back to browse issues page
Downregulation of Matrix Metalloproteinases 2 and 9 is Involved in the Protective Effect of Trehalose on Spinal Cord Injury
Masoumeh Mirzaie 1, Mehrnaz Karimi 1, Hossein Fallah 1, Mohammad Khaksari 2, Mahdieh Nazari-Robati * 3
1- Department of Clinical Biochemistry, Afzalipour School of Medicine, Kerman University of Medical Sciences, Kerman, Iran.
2- Endocrinology and Metabolism Research Center, Institute of Basic and Clinical Physiology Sciences, Kerman University of Medical Sciences, Kerman, Iran.
3- Neuroscience Research Center, Institute of Neuropharmacology and Department of Clinical Biochemistry, Afzalipour School of Medicine, Kerman University of Medical Sciences, Kerman, Iran. , mnazari@kmu.ac.ir
Abstract:   (1141 Views)
Upregulation of matrix metalloproteinases (MMPs), in particular MMP-2 and MMP-9 contributes to secondary pathogenesis of spinal cord injury (SCI) via promoting inflammation. Recently, we reported that trehalose suppresses inflammatory responses following SCI. Therefore, we investigated the effect of trehalose on MMP-2 and MMP-9 expression in SCI. A weight-drop contusion SCI was induced in male rats. Then, animals received trehalose at three doses of 10 (T10), 100 (T100) and 1000 (T1000) mM intrathecally. MMP-2 and MMP-9 transcripts were then measured in damaged spinal cord at 1, 3 and 7 days after trauma, and compared with vehicle and sham groups. Additionally, behavioral analysis was conducted for 1 week using Basso-Beattie-Bresnahan (BBB) locomotor rating scale. Our data showed an early upregulation of MMP-9 at 1 day post-SCI. However, MMP-2 expression was increased at 3 days after trauma. Treatment with 10 mM trehalose significantly reduced MMP-2 expression at 3 and 7 days (P< 0.01) and MMP-9 expression at 1, 3, and 7 days (P< 0.05) post-damage compared with vehicle. However, downregulation of both MMPs was not observed in T100 and T1000 groups. In addition, T10 group showed more rapid recovery of hind limb strength compared with T100 and T1000 groups. We propose that neuroprotective effect of low dose trehalose is mediated by attenuation of MMP-2 and MMP-9 expression.
Keywords: Spinal cord injury, trehalose, matrix metalloproteinases
Full-Text [PDF 283 kb]   (408 Downloads)    
Type of Study: Original Article | Subject: Neurosciences
Received: 2017/12/6 | Accepted: 2018/03/17 | Published: 2018/03/23



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Volume 7, Issue 1 (Int J Mol Cell Med 2018) Back to browse issues page