:: Volume 6, Issue 4 (Int J Mol Cell Med 2017) ::
Int J Mol Cell Med 2017, 6(4): 212-221 Back to browse issues page
Dysregulated Expression of Long Intergenic Non-coding RNAs (LincRNAs) in Urothelial Bladder Carcinoma
Zahra Ousati Ashtiani1, Gholamreza Pourmand1, Seyed Alireza Salami2, Mohsen Ayati3, Javad Tavakkoly Bazzaz 4
1- Urology Research Center, Tehran University of Medical Sciences, Tehran, Iran.
2- Department of Biotechnology, University of Tehran, Tehran, Iran.
3- Uro-Oncology Research Center, Tehran University of Medical Sciences, Tehran, Iran.
4- Department of Medical Genetics, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran. , tavakkolybazzazj@tums.ac.ir
Abstract:   (2455 Views)

Long intergenic non-coding RNA (lincRNA) has been introduced as key regulators of diverse biological processes, including transcription, chromatin organization, cell growth and tumorigenesis. With regard to the potential role of lincRNAs in cancer development, one may postulate that differential expression of lincRNAs could be employed as a tool in cancer diagnosis, prognosis, and targeted therapy. In this study, we aimed to explore the putative correlation between the expression levels of two lincRNAs: LINC00152 and LINC01082 in the bladder cancer (BC), in comparison with its adjacent non-cancerous tissue. Fifty Iranian subjects diagnosed with BC, representing in different stages and grades participated in this study.. The mRNA expression levels of the above mentioned lincRNAs were analyzed comparatively in cancerous and their adjacent non-cancerous counterpart tissues, of each subject by Real-Time PCR. The expression levels of LINC00152, and LINC01082 were significantly lower in tumor tissues in comparison with their adjacent normal tissues (P< 0.001). More notably, in the case of LINC01082 the reduced expression was differentiated by the muscle invasiveness pattern of the tumor (P= 0.05). Our study bestow a new finding about the tumor suppressor potentiality of these lincRNAs in BC development that in turn may suggest them as candidate biomarkers. Replicating this study in higher number of BC subjects, coupled with functional analysis, is necessary to investigate inter-connections between these RNAs and cancer development, leading to better understanding of cancer biology.

Keywords: Bladder cancer, long intergenic non-coding RNA (lincRNA), LINC00152, LINC01082
Full-Text [PDF 440 kb]   (560 Downloads)    
Type of Study: Original Article | Subject: Genetics & Disease
Received: 2017/08/22 | Accepted: 2017/11/12 | Published: 2017/11/12

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Volume 6, Issue 4 (Int J Mol Cell Med 2017) Back to browse issues page