:: Volume 6, Issue 4 (Int J Mol Cell Med (In press) 2017) ::
Int J Mol Cell Med 2017, 6(4): 235-238 Back to browse issues page
Characteristics of Potential Protein Biomarkers Extracted with 10% TCA from Blood Serum of Non-Hodgkin’s Lymphoma and Multiple Myeloma Patients
Severyn Myronovskij 1, Olga Shalay 2, Veronika Spivak 3, Rostyslav Stoika 1, Yuriy Kit * 4
1- Department of Regulation of Cell Proliferation and Apoptosis, Institute of Cell Biology, National Academy of Sciences of Ukraine, Lviv, Ukraine., Department of Regulation of Cell Proliferation and Apoptosis, Institute of Cell Biology, National Academy of Sciences of Ukraine, Lviv, Ukraine.
2- Laboratory of Immunocytology and Genetics of Blood Tumors , Institute of Blood Pathology and Transfusion Medicine, National Academy of Medical Sciences of Ukraine, Lviv, Ukraine., Laboratory of Immunocytology and Genetics of Blood Tumors , Institute of Blood Pathology and Transfusion Medicine, National Academy of Medical Sciences of Ukraine, Lviv, Ukraine.
3- Biological Faculty of Ivan Franko Lviv National University, Lviv, Ukraine., Biological Faculty of Ivan Franko Lviv National University, Lviv, Ukraine.
4- Department of Regulation of Cell Proliferation and Apoptosis, Institute of Cell Biology, National Academy of Sciences of Ukraine, Lviv, Ukraine., Department of Regulation of Cell Proliferation and Apoptosis, Institute of Cell Biology, National Academy of Sciences of Ukraine, Lviv, Ukraine. , kit@cellbiol.lviv.ua
Abstract:   (1514 Views)

Blood serum has been extensively explored as a source of the bio-markers [1, 2].  For concentration of minor protein(s) and depletion of abundant blood serum proteins a 2,2,2-trichloroacetic acid (TCA) precipitation procedure is frequently applied [3]. However, a significant amount of proteins may be present in the TCA extracts, and these proteins are often not studied.Recently, we have shown that a TCA-extracted fraction obtained from blood serum of the multiple sclerosis (MS) patients contains two proteins that were identified by the MALDI TOF/TOF as blood serum albumin (BSA) and a short form of the unconventional myosin lc (sMyo1C) [4]. We also demonstrated that the TCA-extracted fractions isolated from blood serum of the MS patients contain IgGs and/or their heavy chains [5]. These proteins have not been detected in the TCA-extracted fractions isolated from blood serum of healthy human donors and patients with the systemic lupus erythematosus or the rheumatoid arthritis.Here we report that the TCA-soluble fraction isolated from blood serum of the non-Hodgkin’s lymphoma contains sMyo1C that have been earlier detected in blood serum of the MS patients, while the blood serum of the multiple myeloma patients, in addition to that protein and albumin, also contains the IgG polypeptides.

Keywords: blood serum, TCA-extracted proteins, non-Hodgkin’s lymphoma, multiple myeloma, bio-markers.
Full-Text [PDF 414 kb]   (277 Downloads)    
Type of Study: Letters to the editor | Subject: Biomarkers (diagnosis & treatment)
Received: 2017/07/14 | Accepted: 2017/10/7 | Published: 2017/11/11



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Volume 6, Issue 4 (Int J Mol Cell Med (In press) 2017) Back to browse issues page