:: Volume 6, Issue 2 (Int J Mol Cell Med 2017) ::
Int J Mol Cell Med 2017, 6(2): 87-95 Back to browse issues page
Evaluation of MicroRNA-99a and MicroRNA-205 Expression Levels in Bladder Cancer
Sajjad Mohammad Ganji 1, Massoud Saidijam 1, Razeyeh Amini 1, Seyed Habibollah Mousavi-Bahar Seyed2, Nooshin Shabab 1, Saman Seyedabadi 1, Ali Mahdavinezhad *3
1- Research Center for Molecular Medicine, Department of Genetics and Molecular Medicine, Hamadan University of Medical Sciences, Hamadan, Iran., Research Center for Molecular Medicine, Department of Genetics and Molecular Medicine, Hamadan University of Medical Sciences, Hamadan, Iran.
2- Urology and Nephrology Research Center, Shahid Beheshti Hospital, Hamadan University of Medical Sciences, Hamadan, Iran., Urology and Nephrology Research Center, Shahid Beheshti Hospital, Hamadan University of Medical Sciences, Hamadan, Iran.
3- Research Center for Molecular Medicine, Department of Genetics and Molecular Medicine, Hamadan University of Medical Sciences, Hamadan, Iran., Research Center for Molecular Medicine, Department of Genetics and Molecular Medicine, Hamadan University of Medical Sciences, Hamadan, Iran. , alimahdavin@gmail.com
Abstract:   (863 Views)

Bladder cancer is the second most common cancer in the genitourinary tract, showing often recurrence and progresse into invasive states. Epigenetic changes, such as microRNA alteration are involved in bladder cancer tumorigenesis through a variety of signaling pathways. The epigenetic state depends on geographic and lifestyle conditions. The aim of this study was to investigate the expression level of microRNA-99a and microRNA-205 in bladder cancer in Iranian populations and to determine the relationship between their expressions with clinicophatological features. 36 patients with bladder cancer were included in the study. The control group was the healthy adjacent tissue of the same patients. Total RNA was extracted from approximately 50 mg tissue using TRIzol reagent. cDNA was synthesized and Real-Time PCR was carried out using specific primers. The Unisp6 rRNA was used as a reference gene. A significant decrease was found in the expression level of miR-99a in tumor samples, compared to healthy adjacent tissues (P<0.001). The increased expression level of miR-99a was significantly associated with muscle invasion (P=0.02). The receiver operating characteristic (ROC) analysis for miR-99a showed AUC value equal to 0.944, with specificity of 97%, sensitivity of 91%, and cut off value of 8.31 (P<0.001). A significant association was found between smoking and miR-99a (P=0.04) and miR-205 (P= 0.01) expression levels. Dramatic down-regulation of miR-99a in bladder cancer tissues confirmed the tumor suppressor role of miR-99a in bladder cancer. A higher amount of miR-99a expression was associated with invasive bladder cancer. According to ROC analysis, miR-99a could be considered as a valuable diagnostic biomarker.

Keywords: microRNAs, neoplasms, Urinary bladder
Full-Text [PDF 387 kb]   (274 Downloads)    
Type of Study: Original Article | Subject: Cancer
Received: 2017/01/30 | Accepted: 2017/06/17 | Published: 2017/07/9



DOI: 10.22088/acadpub.BUMS.6.2.3


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Volume 6, Issue 2 (Int J Mol Cell Med 2017) Back to browse issues page