:: Volume 6, Issue 2 (Int J Mol Cell Med 2017) ::
Int J Mol Cell Med 2017, 6(2): 96-108 Back to browse issues page
Development of a Novel Anti-Adhesive Vaccine Against Pseudomonas aeruginosa Targeting the C-terminal Disulfide Loop of the Pilin Protein
Sobhan Faezi 1, Ahmad Reza Bahrmand *2, Mehdi Mahdavi 3, Seyed Davar Siadat 1, Iraj Nikokar 4, Soroush Sardari 5
1- Departments of Mycobacteriology and Pulmonary Research, Pasteur Institute of Iran, Tehran, Iran., Departments of Mycobacteriology and Pulmonary Research, Pasteur Institute of Iran, Tehran, Iran.
2- Departments of Mycobacteriology and Pulmonary Research, Pasteur Institute of Iran, Tehran, Iran., Departments of Mycobacteriology and Pulmonary Research, Pasteur Institute of Iran, Tehran, Iran. , Padideh79@yahoo.com
3- Departments of Immunology, Pasteur Institute of Iran, Tehran, Iran., Departments of Immunology, Pasteur Institute of Iran, Tehran, Iran.
4- Laboratory of Microbiology and Immunology of Infectious Diseases, Para Medicine Faculty, Guilan University of Medical Sciences, Rasht, Iran., Laboratory of Microbiology and Immunology of Infectious Diseases, Para Medicine Faculty, Guilan University of Medical Sciences, Rasht, Iran.
5- Biotechnology Research Center, Drug Design and Bioinformatics Group, Pasteur Institute of Iran, Tehran, Iran., Biotechnology Research Center, Drug Design and Bioinformatics Group, Pasteur Institute of Iran, Tehran, Iran.
Abstract:   (1005 Views)

The type IV pili (T4P) is a major virulence factor of Pseudomonas aeruginosa (P. aeruginosa) that is associated with primary adhesion, biofilm formation and twitching motility. This study focuses on the introduction of a novel biologically active subunit vaccine derived from the disulfide loop (DSL) of P. aeruginosa pilin. We investigated the expression of the novel PilA in-frame with pET26a vector, which contains three domains, that each domain contains three tandem repeats. The flexible (GGGGS) and (GGGGS)3 linkers were linked between the three tandem repeats and each pilA domain, respectively.  The recombinant construct (pET26b/pilA) was transformed and expressed in Escherichia coli BL21 (DE3). The reactivity of specific antiserum against PilA was assessed by ELISA method. The biological activities of this candidate vaccine were evaluated by western blotting, opsonophagocytosis and twitching inhibition assays. The pET26b/pilA plasmid was confirmed by enzymatic digestion. The purified PilA protein was confirmed by immunoblot analysis. The checkerboard titration showed that the optimal dilution of the antibody to react with antigen was 1:8. The results of opsonophagocytosis assay revealed that the antibodies raised against PilA promoted phagocytosis of the PAO1 and 6266E strains, to some extent (17.5% and 16.3%, respectively), so that the twitching inhibition test confirmed this result.  Taken together, these are preliminary results based on a first chimerical structure failure in order to induce antibodies that promote the opsonization and eradication of the pathogen. Therefore, the biological activity of the PilA protein showed that it should be introduced with other proteins or target antigens against P. aeruginosa in the future studies.

Keywords: Pseudomonas aeruginosa, type IV pili, disulfide loop, vaccine, pET26b
Full-Text [PDF 658 kb]   (490 Downloads)    
Type of Study: Original Article | Subject: Genomics & Proteomics and Medical Biotechnology
Received: 2017/01/7 | Accepted: 2017/04/3 | Published: 2017/05/31



DOI: 10.22088/acadpub.BUMS.6.2.4


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Volume 6, Issue 2 (Int J Mol Cell Med 2017) Back to browse issues page