:: Volume 6, Number 1 (Int J Mol Cell Med 2017) ::
Int J Mol Cell Med 2017, 6(1): 22-30 Back to browse issues page
Evaluation of Placentalmir-155-5p and Long Non-coding RNA sONE Expression in Patients with Severe Pre-eclampsia
Faezeh Azizi1, Soraya Saleh Gargari2, Sedigheh Asadi Shahmirzadi3, Fatemeh Dodange3, Vahid Amiri4, Reza Mirfakhraie1, Mir Davood Omrani *5
1- Department of Medical Genetics, Faculty of Medicine, ShahidBeheshtiUniversity of Medical Sciences,Tehran, Iran., Department of Medical Genetics, Faculty of Medicine, ShahidBeheshtiUniversity of Medical Sciences,Tehran, Iran.
2- Feto-Maternal Unit, ShohadayeTajrish Hospital, ShahidBeheshti, University of Medical Sciences, Tehran, Iran., Feto-Maternal Unit, ShohadayeTajrish Hospital, ShahidBeheshti, University of Medical Sciences, Tehran, Iran.
3- Feto-Maternal Unit, Mahdiyeh Hospital, ShahidBeheshti, University of Medical Sciences, Tehran, Iran., Feto-Maternal Unit, Mahdiyeh Hospital, ShahidBeheshti, University of Medical Sciences, Tehran, Iran.
4- School of Allied Medical Sciences,ShahidBeheshti, University of Medical Sciences, Tehran, Iran., School of Allied Medical Sciences,ShahidBeheshti, University of Medical Sciences, Tehran, Iran.
5- Department of Medical Genetics, Faculty of Medicine, ShahidBeheshtiUniversity of Medical Sciences,Tehran, Iran., Department of Medical Genetics, Faculty of Medicine, ShahidBeheshtiUniversity of Medical Sciences,Tehran, Iran. , Davood_omrani@yahoo.co.uk
Abstract:   (1432 Views)

It has been well documented that preeclampsia (PE) has a common etiological background, but little is known about its linkage at the molecular level.Non- coding RNAs are critical posttranscriptional regulators ofgene expression.  This study was performed to determine whether PEis associated with alterations in placental non-codingRNAs expression. MicroRNA(miR)-155-5p and long non-coding RNA (lnc)sONE expression, in placentas collected sequentially from 59 patients with PE and 40 normotensive pregnancies were measured using real-time PCR.The relationship between miR-155-5p and lncsONE expressions wasanalyzed statistically. miR-155-5p expression was increased(fold change=1.6, P=0.04), whilelncsONE expression was not significantly changed(fold change=1.1, P=0.68), in placentas from patients compared with control group.miR-155-5p was upregulated in placentas from patients with PEand may have influencedeNOS expression. These findings indicate that miRNA-155-5p may be involved in PE pathogenesis and could be a potential biomarker for this disease.

Keywords: Preeclampsia, miR-155-5p, long non-coding RNA sONE, real time PCR
Full-Text [PDF 712 kb]   (506 Downloads)    
Type of Study: Original Article | Subject: Genetics & Disease
Received: 2016/12/6 | Accepted: 2017/01/8 | Published: 2017/01/17



DOI: 10.22088/acadpub.BUMS.6.1.22


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Volume 6, Number 1 (Int J Mol Cell Med 2017) Back to browse issues page