:: Volume 1, Issue 4 (Int J Mol Cell Med 2012) ::
Int J Mol Cell Med 2012, 1(4): 185-190 Back to browse issues page
Rab11 is required for maintenance of cell shape via βPS integrin mediated cell adhesion in Drosophila
Tanmay Bhuin 1, Jagat Kumar Roy1
1- Cytogenetics Laboratory, Department of Zoology, Banaras Hindu University, Varanasi 221 005, India.
Abstract:   (13898 Views)
In eukaryotes, vesicle trafficking is regulated by the small monomeric GTPases of the Rab protein family. Rab11, (a subfamily of the Ypt/Rab gene family) an evolutionarily conserved, ubiquitously expressed subfamily of small monomeric Rab GTPases, has been implicated in regulating vesicular trafficking through the recycling of endosomal compartment. In an earlier communication, we have shown that Rab11 is required for cell adhesion, maintenance of cell shape and actin-cytoskeleton organization during Drosophila wing development. Here, we report that Rab11 is required for the maintenance of cell shape via βPS integrin mediated cell adhesion. Cuticle preparations of the embryos, when Rab11 is over-expressed or activity of Rab11 is reduced via a double-stranded RNAi line, show dorsal open phenotypes. Immuno-fluorescence and immuno-histochemical analyses on embryos in the same genetic backgrounds also affect the localization of βPS integrins from the adhesion site of leading edge and amnioserosa cells during the dorsal closure stages of embryogenesis as well as the cellular morphology (cell shape) of the lateral epidermal cells.
Keywords: Amnioserosa, cellular morphology, cuticle, dorsal closure, Drosophila, Rab11
Full-Text [PDF 203 kb]   (1698 Downloads)    
Type of Study: Original Article | Subject: Cell Biology
Received: 2013/01/28 | Accepted: 2013/03/18 | Published: 2013/03/18


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Volume 1, Issue 4 (Int J Mol Cell Med 2012) Back to browse issues page