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Effects of Teucrium Polium Aerial Parts extract on oral glucose tolerance tests and pancreas histopathology in Streptozocin-induced diabetic rats
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Mohsen Tatar1   , Durdi Qujeq 2, Farideh Feizi3 , Hadi Parsian4 , Alieh Sohan Faraji3 , Sohrab Halalkhor1 , Roya Abassi1 , Zeinab Abedian4 , Roughayeh Pourbagher4 , Seyed Mohsen Aghajanpour Mir4 , Hamed Mir1 , Nayer Seyfizadeh1 |
1- Department of Biochemistry & Biophysics, Faculty of Medicine, Babol University of Medical Sciences, Babol, Iran.
2- Cellular and Molecular Biology Research Center (CMBRC), Babol University of Medical Sciences, Babol, Iran. , dqujeq@hotmail.com
3- Department of Anatomical Sciences, Faculty of Medicine, Babol University of Medical Sciences, Babol, Iran.
4- Cellular and Molecular Biology Research Center (CMBRC), Babol University of Medical Sciences, Babol, Iran. |
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Abstract: (19876 Views) |
| Teucrium polium can reduce serum glucose. There are few reports in the literature related to this subject and the resolution of this mechanism requires further experiments. The aim of the present study was to evaluate the effects of Teucrium polium aerial parts extracts on oral glucose tolerance tests and pancreas histology in streptozocin-induced diabetic rats. In order to prepare the aqueous concentrate, aerial parts extract was dissolved in distilled water and was boiled for 30 minutes. For the preparation of ethanolic solution, powder was dissolved in ethanol and mixed by a shaker. Diabetic rats were induced with single IP injection of streptozotocin (STZ) at a dose of 50 mg/kg body weight dissolved in normal saline just before use to the 16 hr fast rats. Both groups, diabetic and normal were sacrificed by ether anesthesia. The tissue samples were formalin fixed and paraffin embedded for microscopic examination in accordance with routine laboratory procedures. Blood was collected from the tail vein of the rats. Serum glucose levels were then measured by commercial kits by using a glucose oxidized method. There were no biochemical abnormalities or histological changes in the pancreas of control rats. Post treatment of Teucrium polium aerial parts extract reduced the severity of streptozotocin diabetic pancreases. Our histopathological investigation along with the biochemical evaluations showed a significant effect on histological changes in the pancreas of induced diabetic rats upon Teucrium polium aerial parts extract treatment (P<0.05). |
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| Keywords: Teucrium polium, oral glucose tolerance tests, pancreas histology, streptozocin, diabetic rats |
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Full-Text [PDF 741 kb]
(6363 Downloads)
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Type of Study: Original Article |
Subject:
Clinical Biochemistry
Received: 2012/02/22 | Accepted: 2013/09/14 | Published: 2013/09/14
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