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Adult Stem Cells Properties in Terms of Commitment, Aging and Biological Safety of Grit-Blasted and Acid-Etched Ti Dental Implants Surfaces
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Chiara Gardin1   , Letizia Ferroni1 , Eriberto Bressan2 , Jose Luis Guiraldo3 , Marco Degidi4 , Adriano Piattelli5 , Barbara Zavan 6 |
1- Department of Biomedical Sciences, University of Padua, Padua, Italy.
2- Department of Neurosciences, University of Padua, Padua, Italy.
3- Department of General Dentistry, Faculty of Medicine and Dentistry, University of Murcia, Murcia, Spain.
4- Private Practice, Bologna, Italy.
5- Department of Medical, Oral and Biotechnological Sciences, University of Chieti, Italy.
6- Department of Biomedical Sciences, University of Padua, Padua, Italy. , barbara.zavan@unipd.it |
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Abstract: (10251 Views) |
| Titanium (Ti) is one of the most widely used biomaterials for manufacturing dental implants. The implant surface properties strongly influence osseointegration. The aim of the present study was to in vitro investigate the characteristics of Ti dental implants in terms of mutagenicity, hemocompatibility, biocompatibility, osteoinductivity and biological safety. The Ames test was used to test the mutagenicity of the Ti dental implants, and the hemolysis assay for evaluating their hemocompatibility. Human adipose - derived stem cells (ADSCs) were then seeded onto these implants in order to evaluate their cytotoxicity. Gene expression analyzing with real-time PCR was carried out to investigate the osteoinductivity of the biomaterials. Finally, the genetic stability of the cells cultured onto dental implants was determined by karyotyping. Our results demonstrated that Ti dental implants are not mutagenic, do not cause hemolysis, and are biocompatible. The MTT assay revealed that ADSCs, seeded on Ti dental implants, proliferate up to 30 days in culture. Moreover, ADSCs loaded on Ti dental implants show a substantial expression of some osteoblast specific markers, such as COL1A1, OPN, ALPL, and RUNX2, as well as chromosomal stability after 30 days of culture in a medium without osteogenic factors. In conclusion, the grit-blasted and acid-etched treatment seems to favor the adhesion and proliferation of ADSCs and improve the osteoinductivity of Ti dental implant surfaces. |
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| Keywords: Titanium dental implants, surface properties, adipose-derived stem cells, biocompatibility, osteogenic differentiation |
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Full-Text [PDF 189 kb]
(2858 Downloads)
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Type of Study: Original Article |
Subject:
Hematology
Received: 2014/09/5 | Accepted: 2014/10/18 | Published: 2014/11/2
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