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Dual Effects of Resveratrol on the Expression and Secretion of Angiogenic Factors
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Pegah Kiyamehr1    , Minoo Shahidi 2, Amir Samii1 , Farhad Zaker1 |
1- Department of Hematology and Blood Banking, School of Allied Medical Sciences, Iran University of Medical Sciences, Tehran, Iran.
2- Department of Hematology and Blood Banking, School of Allied Medical Sciences, Iran University of Medical Sciences, Tehran, Iran. , shahidi.minoo@gmail.com |
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Abstract: (1916 Views) |
| Angiogenesis is an essential process in the growth, development and transition of tumors from dormancy to proliferating state. Resveratrol (RSV), as a natural polyphenolic compound, is claimed to be effective in angiogenesis regulation. The aim of this study was to evaluate the impact of RSV on angiogenesis process in human umbilical vein endothelial cells (HUVECs) alone and co-cultured with Jurkat cells. The effects of RSV on HUVECs and Jurkat cells viability and apoptosis were measured by MTT and annexin-V/PI methods. HUVECs were co-cultured with pre-treated Jurkat cells and incubated for 24-72 h. Angiogenesis was also investigated by analyzing the expression of VEGF, VEGFR-2, and IL-8 employing qPCR and ELISA. Treatment with Jurkat cells had no significant effect on apoptosis of HUVECs at low concentrations of RSV (20-40 µM), but higher concentrations (80-160 µM) increased apoptosis. Although RSV significantly reduced VEGFR2 and IL-8 gene expression as well as IL-8 protein concentration in HUVECs, this effect was reversed in the Jurkat-treated HUVECs in the co-culture model. The expression of VEGF in Jurkat cells significantly increased following treatment with RSV. Therefore, RSV had a direct antiangiogenic effect on HUVECs. Unexpectedly its indirect effect was not significant on Jurkat-treated HUVECs in co-culture conditions. The results of our experiments highlight that although RSV may be effective in antiangiogenesis therapy, in some conditions it may favor angiogenesis. Therefore, appropriate concentrations should be achieved to minimize the possible unpredicted effects of RSV. |
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| Keywords: Angiogenesis, resveratrol, co-culture, VEGFR2, VEGF, IL-8 |
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Full-Text [PDF 595 kb]
(1353 Downloads)
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Type of Study: Original Article |
Subject:
Hematology
Received: 2021/12/26 | Accepted: 2022/07/31 | Published: 2022/07/31
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