:: Volume 3, Issue 3 (Int J Mol Cell Med 2014) ::
Int J Mol Cell Med 2014, 3(3): 146-156 Back to browse issues page
Peptone Supplementation of Culture Medium Has Variable Effects on the Productivity of CHO Cells
Fatemeh Davami 1, Lucia Baldi2, Yashas Rajendra2, Florian M. Wurm2
1- Laboratory of Cellular Biotechnology, Ècole Polytechnique Fédérale de Lausanne, Switzerland. , f_davami@pasteur.ac.ir
2- Laboratory of Cellular Biotechnology, Ècole Polytechnique Fédérale de Lausanne, Switzerland.
Abstract:   (5681 Views)
The optimization of cell culture conditions for growth and productivity of recombinant Chinese hamster ovary (CHO) cells is a critical step in biopharmaceutical manufacturing. In the present study, the effects of the timing and amount of peptone feeding of a recombinant CHO cell line grown in a basal medium in serum-free suspension culture were determined for eight peptones of different origin (plant and casein). The amino acid content and the average molecular weight of the peptones chosen were available. In optimized feeding strategies with single peptones, increase 100 % volumetric productivity and 40 % in cell number were achieved. In feeding strategies with two peptones, several combinations stimulated protein productivity more than either peptone alone, depending on the peptone concentration and time of feeding. Some peptones, which did not stimulate productivity when added alone proved to be effective when used in combination. The combined peptones feeding strategies were more effective with peptones of different origin. Our data support the notion that the origin of peptones provides some guidance in identifying the most effective feeding strategies for recombinant CHO cells.
Keywords: Feeding strategy, mammalian cell culture, plant peptones, recombinant proteins, stable CHO cell lines
Full-Text [PDF 291 kb]   (2086 Downloads)    
Type of Study: Original Article | Subject: Infectious disease (Molecular and Cellular aspects)
Received: 2014/06/28 | Accepted: 2014/08/10 | Published: 2014/08/25


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Volume 3, Issue 3 (Int J Mol Cell Med 2014) Back to browse issues page