:: Volume 10, Issue 2 (Int J Mol Cell Med 2021) ::
Int J Mol Cell Med 2021, 10(2): 133-148 Back to browse issues page
The Role of Bone Marrow-Derived Mesenchymal Stromal Cells and Hesperidin in Ameliorating Nephrotoxicity Induced by Cisplatin in Male Wistar Rats
Khalid Mohamed Mazher1 , Osama Mohamed Ahmed2 , Hadeer Abdallah Sayed3 , Taghreed Mohamed Nabil 4
1- Department of Cytology and Histology, Faculty of Veterinary Medicine, Beni-Suef University,Beni-Suef, Egypt.
2- Physiology Division, Zoology Department, Faculty of Science, Beni-Suef University,Beni-Suef, Egypt.
3- Faculty of Veterinary Medicine, Beni-Suef University,Beni-Suef, Egypt.
4- Department of Cytology and Histology, Faculty of Veterinary Medicine, Beni-Suef University,Beni-Suef, Egypt. , tagh_mhm24@yahoo.com
Abstract:   (1567 Views)
Bone marrow-derived mesenchymal stromal cells (BM-MSCs) and antioxidants opened the way for many effective therapeutic experiments against damaged organs like kidneys. Nephrotoxicity is the main complication of chemotherapeutic drugs. Therefore, the present study aimed to investigate the efficacy of BM-MSCs and hesperidin to treat cisplatin-induced nephrotoxicity in rats. Fifty rats were divided into five equal groups of 10 each. Group-I served as a control group, group-II received a single dose of cisplatin (7.5 mg/kg) intraperitoneally to induce nephrotoxicity, group-III received a daily dose of hesperidin (40 mg/kg) orally for four weeks, and on the 5th day cisplatin was administered an hour before hesperidin administration. Group-IV consisted of cisplatin-treated rats that were intravenously injected with 1х106 BM-MSCs cells/rat once per week. Group V contained cisplatin-treated rats that received a combination of hesperidin and BM-MSCs with the same dosage regimes. After four weeks, serum and kidney samples were collected for biochemical, histological, and immunohistochemical examinations were performed. Cisplatin administered rats showed deteriorated biochemical parameters and severe degenerative changes in renal tissue. Both single and combined hesperidin and BM-MSCs treatments restored the renal biochemical parameters. Histologically, the renal tissues significantly improved in the BM-MSCs treated group in comparison with the hesperidin treated group. Moreover, combined treatment (i.e., group V) showed complete restoration of the normal architecture in the renal tissue. Our data suggest that the combined treatment of BM-MSCs and hesperidin has a potent renoprotective efficacy against cisplatin-induced nephrotoxicity rather than the single treatment.
Keywords: Bone marrow-derived mesenchymal stromal cells, cisplatin, hesperidin, immunohistochemistry, nephrotoxicity, p53, apoptosis
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Type of Study: Original Article | Subject: Stem Cell
Received: 2021/03/24 | Accepted: 2021/05/30 | Published: 2021/05/30

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Volume 10, Issue 2 (Int J Mol Cell Med 2021) Back to browse issues page