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:: Volume 3, Issue 2 (Int J Mol Cell Med 2014) ::
Int J Mol Cell Med 2014, 3(2): 61-73 Back to browse issues page
Distribution of HPV Genotypes and Involvement of Risk Factors in Cervical Lesions and Invasive Cervical Cancer: A Study in an Indian Population
Shikha Srivastava1, U P Shahi2, Arti Dibya3, Sadhana Gupta4, Jagat K Roy *5
1- Cytogenetics Laboratory, Department of Zoology, Banaras Hindu University, Varanasi 221005, India., Cytogenetics Laboratory, Department of Zoology, Banaras Hindu University, Varanasi 221005, India.
2- Department of Radiotherapy and Radiation Medicine, Institute of Medical Sciences, Banaras Hindu University, Varanasi 221005, India., Department of Radiotherapy and Radiation Medicine, Institute of Medical Sciences, Banaras Hindu University, Varanasi 221005, India.
3- Indian Railways Cancer Institute and Research Centre, Varanasi, India., Indian Railways Cancer Institute and Research Centre, Varanasi, India.
4- Department of Obstetrics and Gynaecology, Institute of Medical Sciences, Banaras Hindu University, Varanasi 221005, India., Department of Obstetrics and Gynaecology, Institute of Medical Sciences, Banaras Hindu University, Varanasi 221005, India.
5- Cytogenetics Laboratory, Department of Zoology, Banaras Hindu University, Varanasi 221005, India., Cytogenetics Laboratory, Department of Zoology, Banaras Hindu University, Varanasi 221005, India. , jkroy@bhu.ac.in
Abstract:   (5423 Views)
Human papilloma virus (HPV) is considered as the main sexually transmitted etiological agent for the cause and progression of preneoplastic cervical lesions to cervical cancer. This study is discussing the prevalence of HPV and its genotypes in cervical lesions and invasive cervical cancer tissues and their association with various risk factors in women from Varanasi and its adjoining areas in India. A total of 122 cervical biopsy samples were collected from SS Hospital and Indian Railways Cancer Institute and Research Centre, Varanasi and were screened for HPV infection by PCR using primers from L1 consensus region of the viral genome. HPV positive samples were genotyped by type-specific PCR and sequencing. The association of different risk factors with HPV infection in various grades of cervical lesion was evaluated by chi-square test. A total of 10 different HPV genotypes were observed in women with cervicitis, CIN, invasive squamous cell cervical carcinoma and adenocarcinoma. Increased frequency of HPV infection with increasing lesion grade (p=0.002) was observed. HPV16 being the predominant type was found significantly associated with severity of the disease (p=0.03). Various socio- demographic factors other than HPV including high parity (p<0.0001), rural residential area (p<0.0001), elder age (p<0.0001), low socio-economic status (p<0.0001) and women in postmenopausal group (p<0.0001) were also observed to be associated with cervical cancer.These findings show HPV as a direct cause of cervical cancer suggesting urgent need of screening programs and HPV vaccination in women with low socio-economic status and those residing in rural areas.
Keywords: Cervical cancer, clinical- pathological parameters, genotypes, human papillomavirus, socio-demographic factors, rural area
Full-Text [PDF 271 kb]   (1438 Downloads)    
Type of Study: Original Article | Subject: Infectious disease (Molecular and Cellular aspects)
Received: 2014/04/15 | Accepted: 2014/04/26 | Published: 2014/05/6
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Srivastava S, Shahi U P, Dibya A, Gupta S, Roy J K. Distribution of HPV Genotypes and Involvement of Risk Factors in Cervical Lesions and Invasive Cervical Cancer: A Study in an Indian Population. Int J Mol Cell Med. 2014; 3 (2) :61-73
URL: http://ijmcmed.org/article-1-158-en.html


Volume 3, Issue 2 (Int J Mol Cell Med 2014) Back to browse issues page
International Journal of Molecular and Cellular Medicine (IJMCM) International Journal of Molecular and Cellular Medicine (IJMCM)
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