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Modulation of mRNA Expression of Monoacylglycerol Lipase, Diacylglycerol Lipase and Cannabinoid Receptor-1 in Mice Experimentally Infected with T. gondii
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Sahar Rostami-Mansoor1    , Narges Kalantari 2, Tahmine Gorgani-Firouzjaee3 , Salman Ghaffari4 , Maryam Ghasemi-Kasman1 |
1- Cellular and Molecular Biology Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran.
2- Cellular and Molecular Biology Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran. , nfkala@yahoo.com
3- Infectious Diseases and Tropical Medicine Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran.
4- Department of Mycology and Parasitology, School of Medicine, Babol University of Medical Sciences, Babol, Iran. |
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Abstract: (3604 Views) |
| Toxoplasma gondii, an obligate intracellular parasite, infects more than 30% of world's population. This parasite is considered to be neurotropic, and has high tropism for the central nervous system, and potentially induces cryptogenic epilepsy by no clear mechanism. The current study aimed to investigate the alteration of the main components of the endocannabinoid signaling systems in T. gondii-infected mice. For this purpose, the levels of mRNA expression of monoacylglycerol lipase (MAGL), diacylglycerol lipase (DAGL) and cannabinoid receptor-1 (CB1), were measured by quantitative real time PCR.The mRNA expression level of MAGL was increased by ~ 8-fold in the brains of the Toxoplasma-infected group in comparison with non-infected mice (P < 0.0001). The mRNA expression of CB1 gene in the brain of the infected mice was ~ 2 times higher than that measured in control group (P < 0.01). The mRNA expression level of DAGL remained unchanged in the infected mice. Overall a substantial increase in MAGL and CB1 expression without any changes in DAGL, in the brain of infected mice suggests that T. gondii disturbs the endocannabinoid signaling pathways, which are known as neurotransmitter modulators involved in epilepsy. |
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| Keywords: Cannabinoid receptor-1, diacylglycerol lipase, endocannabinoid, monoacylglycerol lipase, Toxoplasma gondii |
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Full-Text [PDF 222 kb]
(1318 Downloads)
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Type of Study: Short Communication |
Subject:
Infectious disease (Molecular and Cellular aspects)
Received: 2021/02/2 | Accepted: 2021/05/15 | Published: 2021/05/30
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