:: Volume 3, Issue 3 (Int J Mol Cell Med 2014) ::
Int J Mol Cell Med 2014, 3(3): 183-189 Back to browse issues page
Hypothalamic Expression of Melanocortin-4 Receptor and Agouti-related Peptide mRNAs During the Estrous Cycle of Rats
Mohammad Reza Zandi1, Mohammad Reza Jafarzadeh Shirazi1, Amin Tamadon 2, Amir Akhlaghi1, Mohammad Saied Salehi1, Ali Niazi3, Ali Moghadam3
1- Department of Animal Sciences, School of Agriculture, Shiraz University, Shiraz, Iran.
2- Transgenic Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran. , amintamaddon@yahoo.com
3- Biotechnology Research Center, School of Agriculture, Shiraz University, Shiraz, Iran.
Abstract:   (6580 Views)
Melanocortin- 4 receptor (MC4R) and agouti- related peptide (AgRP) are involved in energy homeostasis in rats. According to MC4R and AgRP effects on luteinizing hormone (LH) secretion, they may influence the estrous cycle of rats. Therefore, the aim of this study was to investigate the expression of MC4R and AgRP mRNAs at different stages of estrous cycle in the rat’s hypothalamus. The estrous cycle stages (proestrus, estrus, metestrus and diestrus) were determined in 20 adult female rats using vaginal smears. The rats were divided into four equal groups (n=5). Four ovariectomized rats were selected as controls two weeks after surgery. Using real- time PCR, relative expressions (compared to controls) of MC4R and AgRP mRNAs in the hypothalamus of rats were compared in four different groups of estrous cycle. The relative expression of MC4R mRNA in the hypothalamus of female rats during proestrus stage was higher than those in other stages (P=0.001). Despite a lower mean of relative expression of AgRP mRNA at proestrus stage, the relative expression of AgRP mRNA of the four stages of estrous cycle did not differ (P>0.05). In conclusion, changes in the relative expression of MC4R and AgRP mRNAs in four stages of rat estrous cycle indicated a stimulatory role of MC4R in the proestrus and preovulatory stages and an inhibitory role of AgRP in gonadotropin releasing hormone (GnRH) and LH secretions.
Keywords: Melanocortin- 4 receptor, agouti- related peptide, hypothalamus, estrous cycle, rat
Full-Text [PDF 253 kb]   (1783 Downloads)    
Type of Study: Original Article | Subject: Other
Received: 2014/03/6 | Accepted: 2014/07/14 | Published: 2014/07/27


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Volume 3, Issue 3 (Int J Mol Cell Med 2014) Back to browse issues page