[Home ] [Archive]    
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
:: Volume 3, Issue 2 (Int J Mol Cell Med 2014) ::
Int J Mol Cell Med 2014, 3(2): 108-117 Back to browse issues page
Effect of Shiga Toxin And Its Subunits On Cytokine Induction in Different Cell Lines
Neda Moazzezy1, Mana Oloomi *2, Saeid Bouzari1
1- Molecular Biology Unit, Pasteur Institute of Iran, Pasteur Ave. 13164 Tehran, Iran.
2- Molecular Biology Unit, Pasteur Institute of Iran, Pasteur Ave. 13164 Tehran, Iran. , manaoloomi@yahoo.com
Abstract:   (7512 Views)
Shiga toxins (Stxs) are bacterial virulence factors produced by Shigella dysenteriae serotype 1 and Escherichia coli strains. Stxs are critical factors for the development of diseases such as severe bloody diarrhea and hemolytic uremic syndrome. Additionally, Stxs trigger the secretion of pro- inflammatory cytokines and chemokines, particularly in monocytes or macrophages. The inflammatory cytokines result in the modulation of the immune system, local inflammations and enhancement of cytotoxicity. In this study, stimulation of the pro- inflammatory cytokines IL-1α, IL-1β, IL-6, IL-8, and TNF-α was assessed by recombinant Stx (rStx) and its subunits (rStxA and rStxB). Cytokines expression at mRNA level was investigated by Reverse Transcription-Polymerase Chain Reaction (RT-PCR) method in HeLa cells and THP1 monocyte/ macrophage cell lines. After incubation with rStx and its recombinant subunits, the expression of IL-1α, IL- 6 and IL- 8 mRNAs was strongly induced in HeLa cells. In HeLa cells, low expression of IL-1α mRNA was shown by rStxB induction. Furthermore, the expression of IL-1α and IL-1β mRNAs in undifferentiated THP1 cells was only induced by rStx. In differentiated THP1 cells, rStx and its recombinant subunits elicited the expression of IL-1α, IL-1β, IL-8 and IL- 6 mRNAs. On the other hand, expression of TNF-α mRNA was only induced by rStx. Based on the data, the profile of cytokine induction in response to the rStx, and its subunits differs depending on the cell types.
Keywords: Cytokine, heLa cell line, shiga toxin, THP1 cell line
Full-Text [PDF 361 kb]   (1441 Downloads)    
Type of Study: Original Article | Subject: Infectious disease (Molecular and Cellular aspects)
Received: 2014/03/4 | Accepted: 2014/04/27 | Published: 2014/05/21
Send email to the article author

Add your comments about this article
Your username or Email:

CAPTCHA code


XML     Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Moazzezy N, Oloomi M, Bouzari S. Effect of Shiga Toxin And Its Subunits On Cytokine Induction in Different Cell Lines. Int J Mol Cell Med. 2014; 3 (2) :108-117
URL: http://ijmcmed.org/article-1-148-en.html


Volume 3, Issue 2 (Int J Mol Cell Med 2014) Back to browse issues page
International Journal of Molecular and Cellular Medicine (IJMCM) International Journal of Molecular and Cellular Medicine (IJMCM)
Persian site map - English site map - Created in 0.05 seconds with 32 queries by YEKTAWEB 3877