:: Volume 8, Issue 2 (Int J Mol Cell Med 2019) ::
Int J Mol Cell Med 2019, 8(2): 154-161 Back to browse issues page
Migration Gene Expression of Human Umbilical Cord Mesenchymal Stem Cells: A Comparison between Monophosphoryl Lipid A and Supernatant of Lactobacillus acidophilus
Ali Shojaeian1, Ameneh Mehri-Ghahfarrokhi1, Mehdi Banitalebi-Dehkordi 2
1- Department of Molecular Medicine, School of Advanced Technologies, Shahrekord University of Medical Sciences, Shahrekord, Iran.
2- Cellular and Molecular Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, Iran , mehdibanitalebi@gmail.com
Abstract:   (839 Views)
The capacity of human umbilical cord mesenchymal stem cells (hUMSCs)  for migration and homing is very important in regenerative medicine. A detoxified derivative of lipopolysaccharides (LPS) that lacks many of the endotoxic properties of LPS is monophosphoryl lipid A (MPLA).  Effects of MPLA on the induction of MSCs migration, have not yet been studied. Also, studies have shown that supernatant of Lactobacillus acidophilus (SLA) culture medium, can stimulate the proliferation of macrophages and lymphocytes in vitro by affecting the properties of the chemotaxis and angiogenesis. Our present study aimed to understanding of the migration of hUMSCs during treatment with MPLA and LS, separately. HUMSCs were isolated from human umbilical cord and were characterized by investigating surface markers (CD105, CD90, anti-CD29, CD45, and CD34) and their differentiation into adipogenic and osteogenic lineages. HUMSCs were treated with MPLA (10-3 µg/ml) and SLA (3 µl/ml). The morphological changes were not observed in both treated MSCs. Expression levels of migration markers were determined by quantitative reverse transcription PCR analysis on 2, 4, 6 days after treatment. Results showed that VEGF and MMP-2 but not CXCR-4 was increased in the presence of both treatments. Also, SLA led to a decrease and increase of the expression of VLA-4 and VCAM-1, respectively, while MPLA increased both VLA-4 and VCAM-1 expression.Therefore, it can be suggested that MPLA has more prominent results than SLA, but both treatments can probably be considered as an inducing factor in migration.
Keywords: Human umbilical cord mesenchymal stem cells, monophosphoryl lipid A, Lactobacillus acidophilus, VCAM-1
Full-Text [PDF 484 kb]   (192 Downloads)    
Type of Study: Original Article | Subject: Stem Cell
Received: 2019/04/24 | Accepted: 2019/09/10 | Published: 2020/02/5

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Volume 8, Issue 2 (Int J Mol Cell Med 2019) Back to browse issues page