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:: Volume 7, Issue 1 (Int J Mol Cell Med [In press] 2018) ::
Int J Mol Cell Med 2018, 7(1): 24-31 Back to browse issues page
Overexpression of MiR-138 Inhibits Cell Growth and Induces Caspase-mediated Apoptosis in Acute Promyelocytic Leukemia Cell Line
Rima Manafi shabestari 1, Fatemeh Alikarami 1, Davood Bashash 2, Mostafa Paridar 3, Majid Safa * 4
1- Department of Hematology and Blood Banking, Faculty of Allied Medicine, Iran University of Medical Sciences, Tehran, Iran.
2- Department of Hematology and Blood Banking, School of Allied Medical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
3- Ministry of Health and Medical Education, Deputy of Management and Resources Development, Tehran, Iran.
4- Department of Hematology and Blood Banking, Faculty of Allied Medicine, Iran University of Medical Sciences, Tehran, Iran. , safa.m@iums.ac.ir
Abstract:   (655 Views)
Dysregulated expression of miRNAs can play a vital role in pathogenesis of leukemia. The shortened telomere length, and elevated telomerase activity in acute promyelocytic leukemia cells are mainly indicative of extensive proliferative activity. This study aimed to investigate the effect of overexpression of miR-138 on telomerase activity, and cell proliferation of acute promyelocytic leukemia NB4 cells. MiR-138 was overexpressed in NB4 cells using GFP hsa-miR-138-expressing lentiviruses. hTERT mRNA and protein expression levels were assessed by qRT-PCR and  western blot analysis. For evaluation of apoptosis, annexin-V staining and activation of caspases were assessed using flow cytometry and western blot analysis, respectively. Our data demonstrate that overexpression of miR-138 attenuated the hTERT mRNA and protein expression levels. In addition, cell growth was inhibited, and malignant cells underwent caspase mediated-apoptosis in response to miR-138 overexpression. These findings suggest that loss of miR-138 expression may be associated with increased telomerase activity in NB4 cells. Therefore, strategies for up-regulation of miR-138 may result in inhibition of malignant cell growth, and provide a promising therapeutic approach for acute promyelocytic leukemia.
Keywords: Apoptosis, caspase, hTERT, miR-138, poly ADP ribose polymerase (PARP)
Full-Text [PDF 510 kb]   (194 Downloads)    
Type of Study: Original Article | Subject: Hematology
Received: 2018/01/27 | Accepted: 2018/03/26 | Published: 2018/03/31
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Manafi shabestari R, Alikarami F, Bashash D, Paridar M, Safa M. Overexpression of MiR-138 Inhibits Cell Growth and Induces Caspase-mediated Apoptosis in Acute Promyelocytic Leukemia Cell Line. Int J Mol Cell Med. 2018; 7 (1) :24-31
URL: http://ijmcmed.org/article-1-806-en.html


Volume 7, Issue 1 (Int J Mol Cell Med [In press] 2018) Back to browse issues page
International Journal of Molecular and Cellular Medicine (IJMCM) International Journal of Molecular and Cellular Medicine (IJMCM)
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