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:: Volume 5, Number 4 (Int J Mol Cell Med 2016) ::
Back to browse issues page Int J Mol Cell Med 2016, 5(4): 246-54
Should We Perform Semen Analysis, DNA Fragmentation, and Hypo-osmotic Swelling Tests together?
Samaneh Hasanzadeh Keshteli1, Mir Mehrdad Farsi *2, Soraya Khafri3
1- Department of Anatomical Sciences, School of Medicine, Babol University of Medical Sciences, Babol, Iran., Department of Anatomical Sciences, School of Medicine, Babol University of Medical Sciences, Babol, Iran.
2- Department of Anatomical Sciences, School of Medicine, Babol University of Medical Sciences, Babol, Iran., Department of Anatomical Sciences, School of Medicine, Babol University of Medical Sciences, Babol, Iran. , mfarsica@gmail.com
3- Department of Biostatistics and Epidemiology, Babol University of Medical Sciences, Babol, Iran., Department of Biostatistics and Epidemiology, Babol University of Medical Sciences, Babol, Iran.
Abstract:   (394 Views)

Semen analysis, sperm DNA fragmentation (SDF) and hypo-osmotic swelling test (HOST) are usually performed for the evaluation of sperm fertilizing ability. There are some debates over the necessity of SDF and HOST incorporation in male infertility work-up.Semen of 77 men was evaluated by SDF and HOST through three semen analyses. Sperm parameters were arranged into different categories: <5%, 5-15%, >15% for normal morphology; <50%, 50-70%, >70 % for motility; and <10, 10-20, 21-34, 35-50, >50 million/ml for concentration. SDF analysis was performed and values under 30% were assumed to be normal. Normal range of HOST was considered to be >60%.Only normal sperm morphology had significant relationship with DF rate (P<0.001). Normal morphology, motility, and concentration of sperms had significant relationship with HOST (P<0.001, 0.05, and <0.003,respectively). There was a significant negative correlation between sperm morphology and DF rate. The correlations between sperm parameters and percentage of HOST were significantly positive (r: 0.44, 0.19, and 0.32 for morphology, motility, and concentration, respectively). Receiver operating characteristic curve (ROC) showed that sperm morphology is a strong predictor of the rate of DF and HOST (accuracy: 0.74‚ and 0.81, respectively). The best sperm morphology cut off point for DF and HOST rate prediction was 4.5% and 5.5%, respectively.Sperm morphology had significant correlation with DF rate and HOST and is supposed to be a predictor for these tests. Performing these three tests collectively for evaluation of semen samples would not be necessarily required in all cases.

Keywords: Semen analysis, morphology, DNA fragmentation, hypo-osmotic swelling test, IVF
Full-Text [PDF 226 kb]   (185 Downloads)    
Type of Study: Original Article | Subject: Other
Received: 2016/06/30 | Accepted: 2016/09/17 | Published: 2016/11/13
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Hasanzadeh Keshteli S, Farsi M M, Khafri S. Should We Perform Semen Analysis, DNA Fragmentation, and Hypo-osmotic Swelling Tests together?. Int J Mol Cell Med. 2016; 5 (4) :246-54
URL: http://ijmcmed.org/article-1-553-en.html
Back to browse issues page Volume 5, Number 4 (Int J Mol Cell Med 2016)
International Journal of Molecular and Cellular Medicine (IJMCM) International Journal of Molecular and Cellular Medicine (IJMCM)
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