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:: Volume 1, Issue 4 (Int J Mol Cell Med 2012) ::
Int J Mol Cell Med 2012, 1(4): 209-216 Back to browse issues page
Molecular detection of Integron genes and pattern of antibiotic resistance in Pseudomonas aeruginosa strains isolated from intensive care unit, Shahid Beheshti Hospital, North of Iran
Fatemeh Moradian 1, Elaheh Ferdosi Shahandashti 2, Zahra Moulana 3, Massomeh Moradian kouchaksaraei 4, Fariba Asgharpour 5, Ali Mojtahedi 6, Ramazan Rajabnia *7
1- Department of Microbiology Science and Research Branch Islamic Azad University, Guilan, Iran.
2- Department of Microbiology and Immunology, Babol University of Medical Sciences, Babol Iran.
3- Infectious Diseases and Tropical Medicine Research Center, Babol University of Medical Sciences, Babol, Iran.
4- Department of Microbiology Science and Research Branch Islamic Azad University, Fars, Iran.
5- Faculty of Para-Medicine; Babol University of Medical Sciences, Babol, Iran.
6- Department of Microbiology, Faculty of Medicine, Guilan University Campus, Rasht, Iran.
7- Infectious Diseases and Tropical Medicine Research Center, Babol University of Medical Sciences, Babol, Iran. , Ramazan69@yahoo.com
Abstract:   (9486 Views)
Pseudomonas aeruginosa is one of the most important pathogens that causes nosocomial infections and shows high level of antibiotic resistance. Integrons are one of the transposable elements in bacteria and their role in antibiotic resistance has been well demonstrated. The aim of this study was a molecular characterization of the integron genes and the determination of the resistance or sensitivity pattern to ceftizoxime, ceptazidime. cephotaxim, amikacin, ofloxacin, imipenem, cefepime, ticarcillin, gentamicin, ciprofloxacin, cefazolin and ceftriaxone antibiotics in P. aeruginosa strains isolated from Intensive Care Units (ICU), Shahid Beheshti Hospital, North of Iran. This cross-sectional study was performed from 2011 to 2012. Totally, fifty four P. aeruginosa strains were isolated from ICU at Shahid-Beheshti hospital, Babol, North of Iran. The bacteria were diagnosed based on mobility, pigment production, growth in 420 C, oxidase and catalase tests. PCR analysis was carried out to detect integron genes using hep 35 and hep 36 primers. Also, disk diffusion method was performed to evaluate antibiotic susceptibility of the bacteria using ceftizoxime, ceftazidime, cephotaxime, amikacin, ofloxacin, imipenem, cefepime, ticarcillin, gentamicin, ciprofloxacin, cefazolin and ceftriaxone antibacterial reagents. This study revealed that 20 (37%) P. aeruginosa isolates had integron genes. The antibiotic susceptibility test showed that 52 (96.3%) of the isolates were multidrug-resistant. 12 out of 54 isolated bacteria were resistant to all antibiotics tested. All bacteria were resistant to cefepime (100%) and the highest resistance rate was seen to ceftazidime 92.6% fallowed by cefazolin 96.3%. The lowest resistance rate was observed to ciprofloxacin 38.9%, ofloxacin 44.4%, amikacin 46.3% and ticarcillin 48.1%. According to this study, P. aeruginosa isolates showed high level of antibiotic resistance and the presence of integrons in these strains can explain the influence of these genes in resistance creation. There was a significant association between resistance to cefotaxime, amikacin, ofloxacin, imipenem, ticarcillin, gentamicin and the presence of integrons.
Keywords: Pseudomonas aeruginosa, integrons, drug resistance
Full-Text [PDF 227 kb]   (1642 Downloads)    
Type of Study: Original Article | Subject: Infectious disease (Molecular and Cellular aspects)
Received: 2013/01/9 | Accepted: 2013/05/27 | Published: 2013/05/27
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Moradian F, Ferdosi Shahandashti E, Moulana Z, Moradian kouchaksaraei M, Asgharpour F, Mojtahedi A et al . Molecular detection of Integron genes and pattern of antibiotic resistance in Pseudomonas aeruginosa strains isolated from intensive care unit, Shahid Beheshti Hospital, North of Iran. Int J Mol Cell Med. 2012; 1 (4) :209-216
URL: http://ijmcmed.org/article-1-56-en.html
Volume 1, Issue 4 (Int J Mol Cell Med 2012) Back to browse issues page
International Journal of Molecular and Cellular Medicine (IJMCM) International Journal of Molecular and Cellular Medicine (IJMCM)
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