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:: Volume 2, Number 4 (Int J Mol Cell Med 2013) ::
Int J Mol Cell Med 2013, 2(4): 156-163 Back to browse issues page
Sulfated polysaccharides purified from two species of Padina improve collagen and epidermis formation in the rat
Moazameh Kordjazi1, Bahareh Shabanpour1, Ebrahim Zabihi *2, Mohammad Ali Faramarzi3, Farideh Feizi4, Hassan Ahmadi Gavlighi5, Mohammad Amin Feghhi6, Seyed Abbas Hosseini1
1- Department of Fisheries, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran.
2- Cellular and Molecular Biology research Center (CMBRC), Babol University of Medical Sciences, Babol, Iran. , e.zabihi@mubabol.ac.ir
3- Department of Pharmaceutical Biotechnology, Tehran University of Medical Sciences, Tehran, Iran.
4- Cellular and Molecular Biology research Center (CMBRC), Babol University of Medical Sciences, Babol, Iran.
5- Bioprocess Engineering Center, Technical University of Denmark, Lyngby, Denmark.
6- Persian Gulf Biotechnology Park, Persian Gulf Biotechnology Research Center, Qeshm Island, Iran.
Abstract:   (14278 Views)
Sulfated polysaccharides have shown promising effects on wound healing processes along with many other biological activities. The sulfated polysaccharides extracted from two algae species habitats in Persian Gulf were studied in vivo for their effects on collagen formation and epidermal regeneration. The polysaccharides were purified from aqueous extracts of P. tetrastromatica and P. boergesenii using CaCl2 and ethanol precipitation. The sulfate content of each polysaccharide was determined. Two identical wounds (either burn or excision) were made on the back of 4 groups of male Wistar rats (10 rats per group) under anesthesia. The algal polysaccharide ointments (2%) were applied twice daily on one side and the other wound was treated with Eucerin (as control). The rats were sacrificed on day 7 or 14, and then the wound samples were examined for epidermal thickness by light microscope. Furthermore, hydroxyproline content (as a marker of collagen formation) was spectro-photometrically measured. The polysaccharides purified from P. boergesenii had higher sulfate content (32.6±1%) compared to P. tetrastromatica (19±1%). Both algal polysaccharides show some improvements in collagen formation (hydroxyproline content) and epidermal thickness in both wound models compared to the vehicle. The sulfated polysaccharides purified from P. tetrastromatica and P. boergesenii seaweeds are able to induce collagen formation and epidermal regeneration in the two wound models. The superior healing properties of P. boergesenii polysaccharides might be correlated to its higher sulfate content. Both algal polysaccharides are good candidates for wound healing clinical trials.
Keywords: Brown algae, fucoidan, wound healing
Full-Text [PDF 240 kb]   (1350 Downloads)    
Type of Study: Original Article | Subject: Stem Cell
Received: 2013/08/18 | Accepted: 2013/11/24 | Published: 2013/11/24
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Kordjazi M, Shabanpour B, Zabihi E, Faramarzi M A, Feizi F, Ahmadi Gavlighi H, et al . Sulfated polysaccharides purified from two species of Padina improve collagen and epidermis formation in the rat. Int J Mol Cell Med. 2013; 2 (4) :156-163
URL: http://ijmcmed.org/article-1-103-en.html
Volume 2, Number 4 (Int J Mol Cell Med 2013) Back to browse issues page
International Journal of Molecular and Cellular Medicine (IJMCM) International Journal of Molecular and Cellular Medicine (IJMCM)
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